Display options
Cite
Bordin A, Pagano F, Scaccia E, et al. Oral Plaque from Type 2 Diabetic Patients Reduces the Clonogenic Capacity of Dental Pulp-Derived Mesenchymal Stem Cells. Stem Cells Int. 2019;2019:1516746doi: 10.1155/2019/1516746.
Bordin, A., Pagano, F., Scaccia, E., Saccucci, M., Vozza, I., Incerti, N., Polimeni, A., Cavarretta, E., Chimenti, I., & De Falco, E. (2019). Oral Plaque from Type 2 Diabetic Patients Reduces the Clonogenic Capacity of Dental Pulp-Derived Mesenchymal Stem Cells. Stem cells international, 20191516746. https://doi.org/10.1155/2019/1516746
Bordin, Antonella, et al. "Oral Plaque from Type 2 Diabetic Patients Reduces the Clonogenic Capacity of Dental Pulp-Derived Mesenchymal Stem Cells." Stem cells international vol. 2019 (2019): 1516746. doi: https://doi.org/10.1155/2019/1516746
Bordin A, Pagano F, Scaccia E, Saccucci M, Vozza I, Incerti N, Polimeni A, Cavarretta E, Chimenti I, De Falco E. Oral Plaque from Type 2 Diabetic Patients Reduces the Clonogenic Capacity of Dental Pulp-Derived Mesenchymal Stem Cells. Stem Cells Int. 2019 Jan 14;2019:1516746. doi: 10.1155/2019/1516746. eCollection 2019. PMID: 30755774; PMCID: PMC6348930.
Copy Download .nbib Format: NLM
Share it on

Stem Cells Int. 2019 Jan 14;2019:1516746. doi: 10.1155/2019/1516746. eCollection 2019.

Oral Plaque from Type 2 Diabetic Patients Reduces the Clonogenic Capacity of Dental Pulp-Derived Mesenchymal Stem Cells.

Stem cells international

Antonella Bordin, Francesca Pagano, Eleonora Scaccia, Matteo Saccucci, Iole Vozza, Noemi Incerti, Antonella Polimeni, Elena Cavarretta, Isotta Chimenti, Elena De Falco

Affiliations

  1. Department of Medical Surgical Sciences and Biotechnologies, Sapienza University of Rome, C.so della Repubblica 79, 04100 Latina, Italy.
  2. Department of Oral and Maxillo-Facial Sciences, Sapienza University of Rome, Rome, Italy.

PMID: 30755774 PMCID: PMC6348930 DOI: 10.1155/2019/1516746

Abstract

Type 2 diabetes (T2D) is a major metabolic disease and a key epigenetic risk factor for the development of additional clinical complications. Among them, periodontitis (PD), a severe inflammatory disease ascribable to a dysregulated physiology and composition of the oral microbiota, represents one of the most relevant complications. Periodontitis can impact the structure of the tooth and likely the stem and progenitor cell pool, which actively contributes to the periodontal microenvironment and homeostasis. Modifications of the oral plaque play a key role in the etiopathogenesis of PD caused by T2D. However, to what extent the biology of the progenitor pool is affected has still to be elucidated. In this short report, we aimed to explore the biological effects of oral plaque derived from T2D patients with PD in comparison to non-diabetic patients with PD. Oral plaque samples were isolated from T2D and non-diabetic subjects with PD. Dental pulp stem cells (DPSCs), derived from the premolar tooth, were conditioned for 21 days with oral plaque samples and tested for their clonogenic ability. Cultures were also induced to differentiate towards the osteogenic lineage, and ALP and osteocalcin gene expression levels were evaluated by real-time qPCR. Results have shown that the number of clones generated by DPSCs exposed to T2D oral plaque was significantly lower compared to controls (ctl). The multivariate analysis confirmed that the decreased clonogenesis was significantly correlated only with T2D diagnosis. Moreover, the effect of T2D oral plaque was specific to DPSCs. Indicators of osteogenic differentiation were not significantly affected. This study provides a new biological insight into the effects ascribable to T2D in PD.

References

  1. J Periodontol. 1975 Jul;46(7):409-14 - PubMed
  2. J Periodontol. 1992 Apr;63(4):274-9 - PubMed
  3. Diabetes Care. 2005 Jan;28(1):27-32 - PubMed
  4. Cytokine. 2007 Aug;39(2):147-56 - PubMed
  5. Lancet. 2008 Jan 19;371(9608):237-42 - PubMed
  6. J Periodontol. 2008 Apr;79(4):637-46 - PubMed
  7. Tissue Cell. 2008 Aug;40(4):283-92 - PubMed
  8. J Cell Mol Med. 2010 Jun;14(6B):1635-44 - PubMed
  9. J Dent Res. 2011 May;90(5):646-52 - PubMed
  10. Nat Rev Endocrinol. 2011 Jun 28;7(12):738-48 - PubMed
  11. Ann N Y Acad Sci. 2011 Nov;1237:47-52 - PubMed
  12. Cytotherapy. 2013 Oct;15(10):1228-36 - PubMed
  13. Br Dent J. 2013 Jul;215(1):35-9 - PubMed
  14. Cytotechnology. 2015 Jan;67(1):165-74 - PubMed
  15. Braz Oral Res. 2014;28:null - PubMed
  16. Braz Oral Res. 2015;29:null - PubMed
  17. Biomed Res Int. 2015;2015:162439 - PubMed
  18. BMJ Open Diabetes Res Care. 2015 Oct 19;3(1):e000128 - PubMed
  19. Cell Transplant. 2015;24(11):2307-22 - PubMed
  20. PLoS One. 2015 Dec 22;10(12):e0145262 - PubMed
  21. Stem Cells Int. 2016;2016:4709572 - PubMed
  22. World J Diabetes. 2016 Feb 25;7(4):50-66 - PubMed
  23. Nat Rev Dis Primers. 2015 Jul 23;1:15019 - PubMed
  24. Stem Cell Rev. 2016 Oct;12(5):511-523 - PubMed
  25. Curr Stem Cell Res Ther. 2016;12(1):71-79 - PubMed
  26. Stem Cell Res. 2016 Sep;17(2):342-351 - PubMed
  27. Stem Cell Res Ther. 2016 Sep 22;7(1):141 - PubMed
  28. J Appl Oral Sci. 2017 Jan-Feb;25(1):82-89 - PubMed
  29. Cardiovasc Res. 2017 Mar 15;113(4):378-388 - PubMed
  30. Oxid Med Cell Longev. 2017;2017:2712751 - PubMed
  31. Nat Rev Dis Primers. 2017 Jun 22;3:17038 - PubMed
  32. Cytotherapy. 2017 Oct;19(10):1208-1224 - PubMed
  33. Bull Exp Biol Med. 2017 Oct;163(6):718-721 - PubMed
  34. Nat Rev Endocrinol. 2018 Feb;14(2):88-98 - PubMed
  35. Stem Cell Res Ther. 2017 Dec 13;8(1):279 - PubMed
  36. Acta Diabetol. 2018 Jul;55(7):653-667 - PubMed
  37. Front Bioeng Biotechnol. 2018 May 02;6:49 - PubMed
  38. Sci Rep. 2018 Sep 25;8(1):14365 - PubMed

Publication Types