Display options
Share it on

J Photochem Photobiol B. 2018 Nov;188:135-145. doi: 10.1016/j.jphotobiol.2018.09.010. Epub 2018 Sep 12.

Antimicrobial photodynamic therapy alone or in combination with antibiotic local administration against biofilms of Fusobacterium nucleatum and Porphyromonas gingivalis.

Journal of photochemistry and photobiology. B, Biology

Lívia J Tavares, Erica D de Avila, Marlise I Klein, Beatriz H D Panariello, Denise M P Spolidório, Ana Cláudia Pavarina

Affiliations

  1. Department of Dental Materials and Prosthodontics, São Paulo State University (Unesp), School of Dentistry, Araraquara, Rua Humaitá, 1680, 14801-903 Araraquara, SP, Brazil.
  2. Department of Cariology, Operative Dentistry and Dental Public Health, Indiana University School of Dentistry, 1121 W Michigan St, DS406, Indianapolis, IN 46202, USA.
  3. Department of Physiology and Pathology, São Paulo State University (Unesp), School of Dentistry, Araraquara, Rua Humaitá, 1680, 14801-903, Araraquara, SP, Brazil.
  4. Department of Dental Materials and Prosthodontics, São Paulo State University (Unesp), School of Dentistry, Araraquara, Rua Humaitá, 1680, 14801-903 Araraquara, SP, Brazil. Electronic address: [email protected].

PMID: 30267963 DOI: 10.1016/j.jphotobiol.2018.09.010

Abstract

Antimicrobial photodynamic therapy (aPDT) kills several planktonic pathogens. However, the susceptibility of biofilm-derived anaerobic bacteria to aPDT is poorly characterized. Here, we evaluated the effect of Photodithazine (PDZ)-mediated aPDT on Fusobacterium nucleatum and Porphyromonas gingivalis biofilms. In addition, aPDT was tested with metronidazole (MTZ) to explore the potential antimicrobial effect of the treatment. The minimum inhibitory concentration (MIC) of MTZ was defined for each bacterial species. Single-species biofilms of each species were grown on polystyrene plates under anaerobic conditions for five days. aPDT was performed by applying PDZ at concentrations of 50, 75 and 100 mg/L, followed by exposure to 50 J/cm

Copyright © 2018. Published by Elsevier B.V.

Keywords: Anaerobic bacteria; Combined modality therapy; Metronidazole; Photodynamic therapy

Substances

MeSH terms

Publication Types