Circ Res. 1975 Jun;36(6):97-102. doi: 10.1161/01.res.36.6.97.

Dopamine induced relaxation of isolated canine renal, mesenteric, and femoral arteries contracted with prostaglandin F2-alpha.

Circulation research

L I Goldberg, N Toda

PMID: 236842 DOI: 10.1161/01.res.36.6.97

Abstract

The purpose of this investigation was to develop a system for studying the effects of dopamine on isolated blood vessels. Canine renal, mesenteric, and small femoral arteries (less than 1 mm outside diameter) were exposed to phenoxybenzamine 10-5 M for one hour and contracted with prostaglandin F2-alpha. Cumulative concentrations of dopamine ranging from 10-6 to 10-4 M caused dose-related relaxation of the arteries. Propranolol 10-6 M did not affect the relaxation in concentrations which markedly antagonized the effects of isoproterenol. Large femoral arteries (greater than 1 mm outside diameter) did not relax with similar concentrations of dopamine. N-methyldopamine (epinine) produced similar relaxation; 3-methoxytyramine was inactive. Specific antagonism could not be demonstrated by the postulated dopamine antagonists-haloperidol, chlorpromazine, apomorphine, or bulbocapnine-in concentrations up to 10-5 M. Higher concentration of these agents could not be used because they caused the arteries to relax. This study demonstrated that PGF2-alpha-contracted arteries pretreated with phenoxybenzamine are suitable for further investigations of putative dopamine agonists and antagonists.

Substances

• Adrenergic beta-Antagonists
• Dopamine Antagonists
• Prostaglandins F
• Propranolol
• Papaverine
• Isoproterenol
• Deoxyepinephrine
• Dopamine

MeSH terms

• Adrenergic beta-Antagonists / pharmacology
• Animals
• Deoxyepinephrine / pharmacology
• Dogs
• Dopamine / pharmacology
• Dopamine Antagonists
• Dose-Response Relationship, Drug
• Female
• Femoral Artery / drug effects
• In Vitro Techniques
• Isoproterenol / pharmacology
• Male
• Mesenteric Arteries / drug effects
• Papaverine / pharmacology
• Propranolol / pharmacology
• Prostaglandins F / pharmacology
• Renal Artery / drug effects
• Sensory Receptor Cells
• Structure-Activity Relationship
• Vasomotor System / drug effects

Publication Types

• Journal Article
• Research Support, U.S. Gov't, P.H.S.

LinkOut - more resources

• Full Text Sources
  • Atypon
  • Ovid Technologies, Inc.
• Miscellaneous
  • NCI CPTAC Assay Portal