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Sci Data. 2017 Dec 19;4:170195. doi: 10.1038/sdata.2017.195.

Assembly of an early-matured japonica (Geng) rice genome, Suijing18, based on PacBio and Illumina sequencing.

Scientific data

Shou-Jun Nie, Yu-Qiang Liu, Chun-Chao Wang, Shi-Wei Gao, Tian-Tian Xu, Qing Liu, Hui-Lin Chang, Yu-Bao Chen, Peng-Cheng Yan, Wei Peng, Tian-Qing Zheng, Jian-Long Xu, Zhi-Kang Li

Affiliations

  1. Suihua Branch Institute, Heilongjiang Academy of Agricultural Sciences, 420 Gong-Nong West Road, Suihua, Heilongjiang 152000, China.
  2. Institute of Crop Sciences/National Key Facility for Crop Gene Resources and Genetic Improvement, Chinese Academy of Agricultural Sciences, 12 South Zhong-Guan-Cun Street, Beijing 100081, China.
  3. Beijing Computing Center, No. 7 Mid, Fengxian Rd. Yongfeng Industry Base, Beijing 100094, China.
  4. Shenzhen Institute of Breeding for Innovation, Chinese Academy of Agricultural Sciences, Shenzhen 518120, China.

PMID: 29257136 PMCID: PMC5735919 DOI: 10.1038/sdata.2017.195

Abstract

The early-matured japonica (Geng) rice variety, Suijing18 (SJ18), carries multiple elite traits including durable blast resistance, good grain quality, and high yield. Using PacBio SMRT technology, we produced over 25 Gb of long-read sequencing raw data from SJ18 with a coverage of 62×. Using Illumina paired-end whole-genome shotgun sequencing technology, we generated 59 Gb of short-read sequencing data from SJ18 (23.6 Gb from a 200 bp library with a coverage of 59× and 35.4 Gb from an 800 bp library with a coverage of 88×). With these data, we assembled a single SJ18 genome and then generated a set of annotation data. These data sets can be used to test new programs for variation deep mining, and will provide new insights into the genome structure, function, and evolution of SJ18, and will provide essential support for biological research in general.

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