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Freyer JP, Fillak D, Jett JH. Use of xantham gum to suspend large particles during flow cytometric analysis and sorting. Cytometry. 1989;10(6):803-6doi: 10.1002/cyto.990100620.
Freyer, J. P., Fillak, D., & Jett, J. H. (1989). Use of xantham gum to suspend large particles during flow cytometric analysis and sorting. Cytometry, 10(6), 803-6. https://doi.org/10.1002/cyto.990100620
Freyer, J P, et al. "Use of xantham gum to suspend large particles during flow cytometric analysis and sorting." Cytometry vol. 10,6 (1989): 803-6. doi: https://doi.org/10.1002/cyto.990100620
Freyer JP, Fillak D, Jett JH. Use of xantham gum to suspend large particles during flow cytometric analysis and sorting. Cytometry. 1989 Nov;10(6):803-6. doi: 10.1002/cyto.990100620. PMID: 2582972.
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Cytometry. 1989 Nov;10(6):803-6. doi: 10.1002/cyto.990100620.

Use of xantham gum to suspend large particles during flow cytometric analysis and sorting.

Cytometry

J P Freyer, D Fillak, J H Jett

Affiliations

  1. Life Sciences Division, Los Alamos National Laboratory, New Mexico 87545.

PMID: 2582972 DOI: 10.1002/cyto.990100620

Abstract

In this report we describe the use of xantham gum as a biologically inert material for increasing the viscosity of a suspension of cells or particles during flow cytometric analysis and sorting. A 0.1% concentration of xantham gum in culture medium or saline will increase the viscosity approximately 9-fold. For suspensions of multicellular spheroids 100-400 microns in diameter the measured sedimentation velocity was approximately 9 times slower than that in medium alone. Thus, spheroids of 100 microns diameter remain in suspension in 0.1% xantham gum for 66 min, compared to 7.5 min in culture medium. This allows extended periods of sorting without stirring or agitating the sample suspension. The xantham gum solution is noncytotoxic for periods up to 8 h as measured by clonogenicity assay. Xantham gum has the added advantage that the viscosity is significantly reduced when the solution is subjected to shear stress, such as during flow. This technique should be applicable to extended sorting of suspensions of spheroids, plant cells, and other large particles, as well as for analyzing and sorting single cells for extended periods.

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