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Emanuel BS, Balaban G, Boyd JP, et al. N-myc amplification in multiple homogeneously staining regions in two human neuroblastomas. Proc Natl Acad Sci U S A. 1985;82(11):3736-40doi: 10.1073/pnas.82.11.3736.
Emanuel, B. S., Balaban, G., Boyd, J. P., Grossman, A., Negishi, M., Parmiter, A., & Glick, M. C. (1985). N-myc amplification in multiple homogeneously staining regions in two human neuroblastomas. Proceedings of the National Academy of Sciences of the United States of America, 82(11), 3736-40. https://doi.org/10.1073/pnas.82.11.3736
Emanuel, B S, et al. "N-myc amplification in multiple homogeneously staining regions in two human neuroblastomas." Proceedings of the National Academy of Sciences of the United States of America vol. 82,11 (1985): 3736-40. doi: https://doi.org/10.1073/pnas.82.11.3736
Emanuel BS, Balaban G, Boyd JP, Grossman A, Negishi M, Parmiter A, Glick MC. N-myc amplification in multiple homogeneously staining regions in two human neuroblastomas. Proc Natl Acad Sci U S A. 1985 Jun;82(11):3736-40. doi: 10.1073/pnas.82.11.3736. PMID: 2582423; PMCID: PMC397862.
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Proc Natl Acad Sci U S A. 1985 Jun;82(11):3736-40. doi: 10.1073/pnas.82.11.3736.

N-myc amplification in multiple homogeneously staining regions in two human neuroblastomas.

Proceedings of the National Academy of Sciences of the United States of America

B S Emanuel, G Balaban, J P Boyd, A Grossman, M Negishi, A Parmiter, M C Glick

PMID: 2582423 PMCID: PMC397862 DOI: 10.1073/pnas.82.11.3736
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Abstract

Molecular characterization of two human neuroblastoma cell lines has revealed that both contain multiple homogeneously staining regions (HSRs), each representing a chromosome site of N-myc amplification. The newly established cell line CHP-382/JK had two cytogenetically distinct populations with several identical chromosomal abnormalities, indicating a common progenitor cell. Each population had one HSR, one on chromosome 5 at q31-34 and the other on chromosome 2 at q31-32. Chromosomal in situ hybridization with the N-myc probe pNb-1 demonstrated that both HSRs contained amplified copies of N-myc. Southern blot analysis confirmed amplification of N-myc sequences in genomic DNA of CHP-382/JK. Chromosomal features of CHP-382/JK shared with other neuroblastoma cell lines were the deletion of 1p and the presence of extra 17q material. In addition, the cells were highly reactive to monoclonal antibody PI 153/3 used to identify human neuroblastoma. CHP-382/JK cells were further characterized as neuronal cells by the expression of neurotoxin-responsive Na+ channels. Another neuroblastoma cell line, CHP-134, contained a single cell population with three HSRs, one in the short arm of each chromosome 7 and one in the long arm of a chromosome 6. All three HSRs contained amplified copies of N-myc as shown by in situ hybridization with the N-myc probe pNb-1. One of the 7p HSRs was acquired during culture of CHP-134 cells, whereas the 2q HSR of CHP-382/JK was lost. Such findings highlight the continued process of N-myc amplification and transposition in vitro. To our knowledge, amplification of N-myc in multiple HSRs has not been documented previously in neuroblastoma cell lines.

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References

  1. Blood. 1980 Dec;56(6):1141-4 - PubMed
  2. Biochem Biophys Res Commun. 1979 Jun 13;88(3):933-9 - PubMed
  3. Anal Biochem. 1981 Apr;112(2):295-8 - PubMed
  4. Cancer Genet Cytogenet. 1982 Sep;7(1):33-42 - PubMed
  5. J Virol. 1982 Nov;44(2):696-702 - PubMed
  6. J Biol Chem. 1983 Jan 25;258(2):700-2 - PubMed
  7. Cell. 1983 Feb;32(2):579-88 - PubMed
  8. Cell. 1983 Apr;32(4):1257-67 - PubMed
  9. Proc Natl Acad Sci U S A. 1983 Jul;80(13):4069-73 - PubMed
  10. Cancer Res. 1983 Sep;43(9):4159-66 - PubMed
  11. Nature. 1983 Sep 15-21;305(5931):245-8 - PubMed
  12. Annu Rev Biochem. 1983;52:301-54 - PubMed
  13. Carbohydr Res. 1983 Aug 16;120:197-213 - PubMed
  14. Exp Cell Res. 1983 Oct;148(1):21-30 - PubMed
  15. Nature. 1983 Dec 1-7;306(5942):494-7 - PubMed
  16. Cell. 1983 Dec;35(2 Pt 1):359-67 - PubMed
  17. Science. 1984 Feb 17;223(4637):653-61 - PubMed
  18. Nature. 1984 Mar 15-21;308(5956):288-91 - PubMed
  19. Proc Natl Acad Sci U S A. 1984 Apr;81(8):2444-6 - PubMed
  20. Science. 1984 Jun 8;224(4653):1121-4 - PubMed
  21. Nature. 1984 May 31-Jun 6;309(5967):418-25 - PubMed
  22. Nature. 1984 May 31-Jun 6;309(5967):458-60 - PubMed
  23. Cancer Res. 1984 Nov;44(11):5444-9 - PubMed
  24. Cytogenet Cell Genet. 1984;38(4):308-9 - PubMed
  25. Cancer. 1971 Feb;27(2):374-8 - PubMed
  26. J Mol Biol. 1975 Nov 5;98(3):503-17 - PubMed
  27. Cancer Res. 1976 Sep;36(9 pt.1):3094-100 - PubMed
  28. Biochem Biophys Res Commun. 1976 Sep 7;72(1):209-15 - PubMed
  29. Science. 1977 Nov 18;198(4318):739-41 - PubMed
  30. Cell. 1979 Jan;16(1):201-11 - PubMed
  31. Science. 1979 Mar 16;203(4385):1120-1 - PubMed
  32. J Biol Chem. 1980 Dec 25;255(24):11914-21 - PubMed

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