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D'Amario D, Leone AM, Narducci ML, et al. Human cardiac progenitor cells with regenerative potential can be isolated and characterized from 3D-electro-anatomic guided endomyocardial biopsies. Int J Cardiol. 2017;241:330-343doi: 10.1016/j.ijcard.2017.02.106.
D'Amario, D., Leone, A. M., Narducci, M. L., Smaldone, C., Lecis, D., Inzani, F., Luciani, M., Siracusano, A., La Neve, F., Manchi, M., Pelargonio, G., Perna, F., Bruno, P., Massetti, M., Pitocco, D., Cappetta, D., Esposito, G., Urbanek, K., De Angelis, A., Rossi, F., Piacentini, R., Angelini, G., Li Puma, D. D., Grassi, C., De Paolis, E., Capoluongo, E., Silvestri, V., Merlino, B., Marano, R., & Crea, F. (2017). Human cardiac progenitor cells with regenerative potential can be isolated and characterized from 3D-electro-anatomic guided endomyocardial biopsies. International journal of cardiology, 241330-343. https://doi.org/10.1016/j.ijcard.2017.02.106
D'Amario, Domenico, et al. "Human cardiac progenitor cells with regenerative potential can be isolated and characterized from 3D-electro-anatomic guided endomyocardial biopsies." International journal of cardiology vol. 241 (2017): 330-343. doi: https://doi.org/10.1016/j.ijcard.2017.02.106
D'Amario D, Leone AM, Narducci ML, Smaldone C, Lecis D, Inzani F, Luciani M, Siracusano A, La Neve F, Manchi M, Pelargonio G, Perna F, Bruno P, Massetti M, Pitocco D, Cappetta D, Esposito G, Urbanek K, De Angelis A, Rossi F, Piacentini R, Angelini G, Li Puma DD, Grassi C, De Paolis E, Capoluongo E, Silvestri V, Merlino B, Marano R, Crea F. Human cardiac progenitor cells with regenerative potential can be isolated and characterized from 3D-electro-anatomic guided endomyocardial biopsies. Int J Cardiol. 2017 Aug 15;241:330-343. doi: 10.1016/j.ijcard.2017.02.106. Epub 2017 Mar 01. PMID: 28343765.
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Int J Cardiol. 2017 Aug 15;241:330-343. doi: 10.1016/j.ijcard.2017.02.106. Epub 2017 Mar 01.

Human cardiac progenitor cells with regenerative potential can be isolated and characterized from 3D-electro-anatomic guided endomyocardial biopsies.

International journal of cardiology

Domenico D'Amario, Antonio Maria Leone, Maria Lucia Narducci, Costantino Smaldone, Dalgisio Lecis, Frediano Inzani, Marco Luciani, Andrea Siracusano, Federica La Neve, Melissa Manchi, Gemma Pelargonio, Francesco Perna, Piergiorgio Bruno, Massimo Massetti, Dario Pitocco, Donato Cappetta, Grazia Esposito, Konrad Urbanek, Antonella De Angelis, Francesco Rossi, Roberto Piacentini, Giulia Angelini, Domenica Donatella Li Puma, Claudio Grassi, Elisa De Paolis, Ettore Capoluongo, Valentina Silvestri, Biagio Merlino, Riccardo Marano, Filippo Crea

Affiliations

  1. Department of Cardiovascular Medicine, Catholic University of the Sacred Heart, Rome, Italy. Electronic address: [email protected].
  2. Department of Cardiovascular Medicine, Catholic University of the Sacred Heart, Rome, Italy.
  3. Department of Pathology, Catholic University of the Sacred Heart, Rome, Italy.
  4. Institute of Internal Medicine and Diabetes Care Unit, Catholic University of the Sacred Heart, Rome, Italy.
  5. Department of Experimental Medicine, Section of Pharmacology, Università della Campania "Luigi Vanvitelli", Naples, Italy.
  6. Institute of Human Physiology, Catholic University of the Sacred Heart, Rome, Italy.
  7. Institute of Human Physiology, Catholic University of the Sacred Heart, Rome, Italy; San Raffaele Pisana Scientific Institute for Research, Hospitalization and Health Care, Rome, Italy.
  8. Laboratory of Molecular Biology, Institute of Biochemistry and Clinical Biochemistry, Catholic University of the Sacred Heart, Rome, Italy.
  9. Department of Radiological Sciences, Institute of Radiology, Catholic University of the Sacred Heart, Rome, Italy.
  10. Department of Cardiovascular Medicine, Catholic University of the Sacred Heart, Rome, Italy. Electronic address: [email protected].

PMID: 28343765 DOI: 10.1016/j.ijcard.2017.02.106

Abstract

AIMS: In the present study, we aimed to develop a percutaneous approach and a reproducible methodology for the isolation and expansion of Cardiac Progenitor Cells (CPCs) from EndoMyocardial Biopsies (EMB) in vivo. Moreover, in an animal model of non-ischemic heart failure (HF), we would like to test whether CPCs obtained by this methodology may engraft the myocardium and differentiate.

METHODS AND RESULTS: EMB were obtained using a preformed sheath and a disposable bioptome, advanced via right femoral vein in 12 healthy mini pigs, to the right ventricle. EMB were enzymatically dissociated, cells were expanded and sorted for c-kit. We used 3D-Electro-Anatomic Mapping (3D-EAM) to obtain CPCs from 32 patients affected by non-ischemic cardiomyopathy. The in vivo regenerative potential of CPCs was tested in a rodent model of drug-induced non-ischemic cardiomyopathy. c-kit positive CPCs replicative capacity was assessed in 30 patients. Telomere length averaged 7.4±0.4kbp and telomerase activity was present in all preparations (1.7×10

CONCLUSIONS: The success in obtaining CPCs characterized by high regenerative potential, in vitro and in vivo, from EMB indicates that harvesting without thoracotomy in patients affected by either ischemic or non-ischemic cardiomyopathy is feasible. These initial results may potentially expand the future application of CPCs to all patients affected by HF not undergoing surgical procedures.

Copyright © 2017 Elsevier B.V. All rights reserved.

Keywords: 3D-endomyocardial biopsies; Cardiac progenitor/stem cells; Endomyocardial biopsies; Heart failure

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