J Anal Toxicol. 1989 Nov-Dec;13(6):313-6. doi: 10.1093/jat/13.6.313.

Simultaneous high-performance liquid chromatographic analysis of hippuric acid and ortho-, meta-, and para-methylhippuric acids in urine.

Journal of analytical toxicology

R Tardif, J Brodeur, G L Plaa

PMID: 2607759 DOI: 10.1093/jat/13.6.313

Abstract

A high-performance liquid chromatographic assay for the simultaneous analysis of hippuric and methylhippuric acids in urine is described. Compounds are first extracted from acidified urines after addition of o-methylbenzoylalanine (internal standard), using methyl-t-butyl ether. The organic phase is evaporated under nitrogen flow and the residue dissolved in the mobile phase which consisted of 91% potassium phosphate buffer (12.0mM, pH: 2.0), 4.5% methanol, and 4.5% tetrahydrofuran. The method described allows complete separation of meta- and para-methylhippuric acids in less than 20 min using a stainless steel column packed with octadecyl-dimethysilyl silica. The authors used this method to determine the urinary excretion of hippuric and methylhippuric acids in rabbits following exposure to a mixture of toluene and isomers of xylene. Data indicated that under the actual experimental conditions of exposure o-xylene is excreted to a lesser extent as a methylhippuric acid than the meta- and para-xylenes.

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Substances

• Hippurates
• 3-methylhippuric acid
• 4-methylhippuric acid
• 2-methylhippuric acid
• hippuric acid

MeSH terms

• Animals
• Chromatography, High Pressure Liquid / methods
• Hippurates / urine
• Rabbits

Publication Types

• Journal Article
• Research Support, Non-U.S. Gov't

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