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Stanton R, Hykollari A, Eckmair B, et al. The underestimated N-glycomes of lepidopteran species. Biochim Biophys Acta Gen Subj. 2017;1861(4):699-714doi: 10.1016/j.bbagen.2017.01.009.
Stanton, R., Hykollari, A., Eckmair, B., Malzl, D., Dragosits, M., Palmberger, D., Wang, P., Wilson, I. B., & Paschinger, K. (2017). The underestimated N-glycomes of lepidopteran species. Biochimica et biophysica acta. General subjects, 1861(4), 699-714. https://doi.org/10.1016/j.bbagen.2017.01.009
Stanton, Rhiannon, et al. "The underestimated N-glycomes of lepidopteran species." Biochimica et biophysica acta. General subjects vol. 1861,4 (2017): 699-714. doi: https://doi.org/10.1016/j.bbagen.2017.01.009
Stanton R, Hykollari A, Eckmair B, Malzl D, Dragosits M, Palmberger D, Wang P, Wilson IB, Paschinger K. The underestimated N-glycomes of lepidopteran species. Biochim Biophys Acta Gen Subj. 2017 Apr;1861(4):699-714. doi: 10.1016/j.bbagen.2017.01.009. Epub 2017 Jan 08. PMID: 28077298; PMCID: PMC5330436.
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Biochim Biophys Acta Gen Subj. 2017 Apr;1861(4):699-714. doi: 10.1016/j.bbagen.2017.01.009. Epub 2017 Jan 08.

The underestimated N-glycomes of lepidopteran species.

Biochimica et biophysica acta. General subjects

Rhiannon Stanton, Alba Hykollari, Barbara Eckmair, Daniel Malzl, Martin Dragosits, Dieter Palmberger, Ping Wang, Iain B H Wilson, Katharina Paschinger

Affiliations

  1. Department für Chemie, Universität für Bodenkultur, 1190 Wien, Austria.
  2. Department für Biotechnologie, Universität für Bodenkultur, 1190 Wien, Austria.
  3. Department of Entomology, Cornell University, Geneva, NY 14456, USA.
  4. Department für Chemie, Universität für Bodenkultur, 1190 Wien, Austria. Electronic address: [email protected].

PMID: 28077298 PMCID: PMC5330436 DOI: 10.1016/j.bbagen.2017.01.009

Abstract

BACKGROUND: Insects are significant to the environment, agriculture, health and biotechnology. Many of these aspects display some relationship to glycosylation, e.g., in case of pathogen binding or production of humanised antibodies; for a long time, it has been considered that insect N-glycosylation potentials are rather similar and simple, but as more species are glycomically analysed in depth, it is becoming obvious that there is indeed a large structural diversity and interspecies variability.

METHODS: Using an off-line LC-MALDI-TOF MS approach, we have analysed the N-glycomes of two lepidopteran species (the cabbage looper Trichoplusia ni and the gypsy moth Lymantria dispar) as well as of the commonly-used T. ni High Five cell line.

RESULTS: We detected not only sulphated, glucuronylated, core difucosylated and Lewis-like antennal fucosylated structures, but also the zwitterion phosphorylcholine on antennal GlcNAc residues, a modification otherwise familiar from nematodes; in L. dispar, N-glycans with glycolipid-like antennae containing α-linked N-acetylgalactosamine were also revealed.

CONCLUSION: The lepidopteran glycomes analysed not only display core α1,3-fucosylation, which is foreign to mammals, but also up to 5% anionic and/or zwitterionic glycans previously not found in these species.

SIGNIFICANCE: The occurrence of anionic and zwitterionic glycans in the Lepidoptera data is not only of glycoanalytical and evolutionary interest, but is of biotechnological relevance as lepidopteran cell lines are potential factories for recombinant glycoprotein production.

Copyright © 2017 Elsevier B.V. All rights reserved.

Keywords: Glucuronic acid; Insect; N-linked oligosaccharides; Phosphorylcholine; Sulphate

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