BMC Infect Dis. 2016 Aug 19;16(1):429. doi: 10.1186/s12879-016-1778-6.
Impact of aging on TREM-1 responses in the periodontium: a cross-sectional study in an elderly population.
BMC infectious diseases
Veli Özgen Öztürk, Georgios N Belibasakis, Gülnur Emingil, Nagihan Bostanci
Affiliations
Affiliations
- Department of Periodontology, School of Dentistry, Adnan Menderes University, Ayd?n, Turkey.
- Department of Dental Medicine, Karolinska Institute, Stockholm, Sweden.
- Department of Periodontology, School of Dentistry, Ege University, Izmir, Turkey.
- Department of Dental Medicine, Karolinska Institute, Stockholm, Sweden. [email protected].
PMID: 27542376
PMCID: PMC4992242 DOI: 10.1186/s12879-016-1778-6
Abstract
BACKGROUND: Aging is associated with altered immune response, which increases susceptibility to infections. sTREM-1 is involved in the amplification of the inflammatory response to bacterial infection. The present cross-sectional study aims to investigate local sTREM-1 levels in gingival crevicular fluid (GCF) as well as key periodontal pathogen levels in the subgingival plaque in an elderly cohort with periodontal health, gingivitis, and chronic periodontitis (CP).
METHODS: Subjects were 51 systemically healthy, elderly individuals (mean age, 68 ± 4.5 years) who had undergone full-mouth periodontal examinations. Subgingival plaque and GCF samples were collected from the healthy sites of participants without periodontal disease (n = 17), the sites with gingival inflammation from patients with gingivitis (n = 19), and the periodontitis sites of patients with CP (n = 15). GCF volumes were measured by an electronic impedance device, and total protein levels were assessed by a flouremetric assay. sTREM-1 levels in GCF were measured by enzyme-linked immunosorbent assay. The subgingival plaque total bacteria, Porphyromonas gingivalis, Tannerella forsythia, Treponema denticola, Fusobacterium nucleatum, and Prevotella intermedia levels were determined by quantitative real-time polymerase chain reaction. Statistical analysis was performed using nonparametric methods.
RESULTS: GCF volume, total protein concentrations, and sTREM-1 levels in GCF were similar among the groups (p > 0.05). Significantly higher T. forsythia levels were observed in subgingival plaque samples harvested from patients with gingivitis and CP, than in those from healthy participants (p < 0.05). However, the subgingival levels of the other four periodontal pathogens and total bacteria were not statistically different among the groups (p > 0.05).
CONCLUSIONS: Our findings suggest that there are no differences in GCF volume, total protein, and sTREM-1 levels between healthy and periodontally diseased elderly adults. We found only limited differences in the studied subgingival microbial profile. This finding indicates an already deregulated, local inflammatory response in this elderly cohort, on which bacterial biofilm challenge may have a limited further impact.
Keywords: Aging; Elderly; Gingival crevicular fluid; Subgingival plaque; sTREM-1
References
- Nat Immunol. 2006 Dec;7(12):1266-73 - PubMed
- PLoS One. 2013 Oct 04;8(10):e75784 - PubMed
- BMC Infect Dis. 2012 Jul 18;12 :157 - PubMed
- J Periodontol. 1967 Nov-Dec;38(6):Suppl:610-6 - PubMed
- Ann Rheum Dis. 2009 Nov;68(11):1768-74 - PubMed
- Periodontol 2000. 2014 Feb;64(1):7-19 - PubMed
- J Oral Sci. 2011 Sep;53(3):333-9 - PubMed
- J Clin Periodontol. 2014 Oct;41(10 ):935-42 - PubMed
- Periodontol 2000. 2010 Jun;53:70-88 - PubMed
- Periodontol 2000. 2010 Oct;54(1):78-105 - PubMed
- Exp Gerontol. 2008 Aug;43(8):718-28 - PubMed
- Dig Dis Sci. 2009 Jul;54(7):1525-31 - PubMed
- J Periodontol. 2009 Mar;80(3):436-46 - PubMed
- FEBS Lett. 2007 Mar 20;581(6):1173-8 - PubMed
- Nature. 2001 Apr 26;410(6832):1103-7 - PubMed
- Periodontol 2000. 1997 Jun;14:33-53 - PubMed
- J Periodontal Res. 2015 Feb;50(1):89-102 - PubMed
- Mol Immunol. 2011 Oct;49(1-2):387-94 - PubMed
- Crit Care Med. 2008 Jul;36(7):2048-53 - PubMed
- J Clin Periodontol. 2012 Dec;39(12):1141-8 - PubMed
- Eur J Med Res. 2003 Nov 12;8(11):499-504 - PubMed
- Proc Natl Acad Sci U S A. 1989 Mar;86(6):2018-20 - PubMed
- J Periodontol. 2014 Jan;85(1):204-10 - PubMed
- J Immunol. 2002 Nov 1;169(9):4697-701 - PubMed
- Clin Med Res. 2004 Aug;2(3):181-7 - PubMed
- J Periodontol. 1997 Oct;68(10):973-81 - PubMed
- FEBS Lett. 2005 Apr 11;579(10):2035-9 - PubMed
- J Infect Dis. 2003 Jun 15;187 Suppl 2:S397-401 - PubMed
- J Immunol. 2000 May 15;164(10):4991-5 - PubMed
- Age (Dordr). 2008 Mar;30(1):11-9 - PubMed
- J Clin Periodontol. 1998 Feb;25(2):134-44 - PubMed
- Future Microbiol. 2009 Mar;4(2):155-7 - PubMed
- J Int Oral Health. 2014 Nov-Dec;6(6):110-6 - PubMed
- Gerodontology. 2015 Jun;32(2):132-40 - PubMed
- Mol Oral Microbiol. 2010 Feb;25(1):25-37 - PubMed
- Ann Periodontol. 1999 Dec;4(1):1-6 - PubMed
- J Periodontal Res. 2013 Aug;48(4):517-26 - PubMed
- J Immunol. 2003 Apr 1;170(7):3812-8 - PubMed
- J Dent Res. 2013 Feb;92(2):161-5 - PubMed
- FEMS Immunol Med Microbiol. 2012 Oct;66(1):37-44 - PubMed
- J Clin Periodontol. 2005;32 Suppl 6:57-71 - PubMed
- J Clin Microbiol. 1992 Sep;30(9):2225-9 - PubMed
- Mech Ageing Dev. 2009 Aug;130(8):538-46 - PubMed
Substances
MeSH terms
Publication Types