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Jabbour Z, do Nascimento C, El-Hakim M, et al. Profile of bacteria colonizing the exposed bone of patients with anti-osteoclastic drug-related osteonecrosis of the jaws. Can J Microbiol. 2016;62(9):772-80doi: 10.1139/cjm-2016-0212.
Jabbour, Z., do Nascimento, C., El-Hakim, M., Henderson, J. E., & de Albuquerque, R. F. (2016). Profile of bacteria colonizing the exposed bone of patients with anti-osteoclastic drug-related osteonecrosis of the jaws. Canadian journal of microbiology, 62(9), 772-80. https://doi.org/10.1139/cjm-2016-0212
Jabbour, Zaher, et al. "Profile of bacteria colonizing the exposed bone of patients with anti-osteoclastic drug-related osteonecrosis of the jaws." Canadian journal of microbiology vol. 62,9 (2016): 772-80. doi: https://doi.org/10.1139/cjm-2016-0212
Jabbour Z, do Nascimento C, El-Hakim M, Henderson JE, de Albuquerque RF. Profile of bacteria colonizing the exposed bone of patients with anti-osteoclastic drug-related osteonecrosis of the jaws. Can J Microbiol. 2016 Sep;62(9):772-80. doi: 10.1139/cjm-2016-0212. Epub 2016 May 11. PMID: 27419922.
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Can J Microbiol. 2016 Sep;62(9):772-80. doi: 10.1139/cjm-2016-0212. Epub 2016 May 11.

Profile of bacteria colonizing the exposed bone of patients with anti-osteoclastic drug-related osteonecrosis of the jaws.

Canadian journal of microbiology

Zaher Jabbour, Cássio do Nascimento, Michel El-Hakim, Janet E Henderson, Rubens F de Albuquerque

Affiliations

  1. a Division of Restorative Dentistry, Faculty of Dentistry, McGill University, Montréal, Quebec, Canada.
  2. d Bone Engineering Labs, Research Institute, McGill University Health Center, Montréal, Quebec, Canada.
  3. b Department of Dental Materials and Prosthodontics, Faculty of Dentistry of Ribeirão Preto, University of São Paulo, Brazil.
  4. c Division of Oral and Maxillofacial Surgery, Faculty of Dentistry, McGill University, Montréal, Quebec, Canada.
  5. e Department of Medicine and Surgery, Faculty of Medicine, McGill University, Montréal, Quebec, Canada.

PMID: 27419922 DOI: 10.1139/cjm-2016-0212

Abstract

Microbial etiology for anti-osteoclastic drug-related osteonecrosis of the jaw (ARONJ) was suggested. This study investigates any link between bacteria colonizing ARONJ sites and other oral cavity sites. Microbiota samples of 10 ARONJ patients were collected from the exposed bone, adjacent teeth, contralateral teeth, and tongue. DNA checkerboard hybridization was used for microbiota analysis with 43 genomic DNA probes prepared from human oral bacterial (38) and candida (5) species, using Socransky's bacterial complexes as a guide. The frequency and the mean proportion of each bacterial species were used. Eikenella corrodens, Streptococcus constellatus, and Fusobacterium nucleatum were dominant in the ARONJ sites and detected in most teeth samples. Staphylococcus aureus was also dominant in the ARONJ sites and tongue. Significant correlations were found between the mean proportions of bacterial species colonizing adjacent teeth, contralateral teeth, and tongue (p < 0.001, R(2) > 0.69). No significant correlation (p > 0.05, R(2) < 0.025) was found between bacteria colonizing ARONJ sites and other evaluated sites. Within the study limitations, it was concluded that the primary sources of microorganisms colonizing ARONJ sites could be other sites such as teeth and tongue. The microbial profile of the necrotic bone is predominantly colonized with bacteria from Socransky's green and orange complexes, as well as with species associated with bone infections.

Keywords: DNA checkerboard; anti-osteoclastic drug; bacteria biofilm; biofilm bactérien; cancer; damier d’ADN; jaw osteonecrosis; médicament anti-ostéoclastique; ostéonécrose de la mâchoire

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