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Cohly HH, Morrison DR, Atassi MZ. Antigen presentation by non-immune B-cell hybridoma clones: presentation of synthetic antigenic sites reveals clones that exhibit no specificity and clones that present only one epitope. Immunol Invest. 1989;18(8):987-92doi: 10.3109/08820138909045785.
Cohly, H. H., Morrison, D. R., & Atassi, M. Z. (1989). Antigen presentation by non-immune B-cell hybridoma clones: presentation of synthetic antigenic sites reveals clones that exhibit no specificity and clones that present only one epitope. Immunological investigations, 18(8), 987-92. https://doi.org/10.3109/08820138909045785
Cohly, H H, et al. "Antigen presentation by non-immune B-cell hybridoma clones: presentation of synthetic antigenic sites reveals clones that exhibit no specificity and clones that present only one epitope." Immunological investigations vol. 18,8 (1989): 987-92. doi: https://doi.org/10.3109/08820138909045785
Cohly HH, Morrison DR, Atassi MZ. Antigen presentation by non-immune B-cell hybridoma clones: presentation of synthetic antigenic sites reveals clones that exhibit no specificity and clones that present only one epitope. Immunol Invest. 1989 Oct;18(8):987-92. doi: 10.3109/08820138909045785. PMID: 2479600.
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Taylor & Francis

Immunol Invest. 1989 Oct;18(8):987-92. doi: 10.3109/08820138909045785.

Antigen presentation by non-immune B-cell hybridoma clones: presentation of synthetic antigenic sites reveals clones that exhibit no specificity and clones that present only one epitope.

Immunological investigations

H H Cohly, D R Morrison, M Z Atassi

Affiliations

  1. NASA Johnson Space Center, Houston, Texas 77058.

PMID: 2479600 DOI: 10.3109/08820138909045785

Abstract

Recently, we reported the preparation and antigen-presenting properties of hybridoma B-cell clones obtained after fusing non-secreting, non-antigen presenting Balb/c 653-myeloma cells with non-immune SJL spleen cells. It was found that antigen presentation at the clonal level can be specific or non-specific, depending on the particular B-cell clone. In the present work, one specific and one general presenter B-cell clones were tested for their epitope presentation ability to SJL T-cells that were specific to lysozyme or myoglobin. B-cell clone A1G12, a general presenter which presented both lysozyme and myoglobin to their respective T-cell lines, was found to present all five myoglobin epitopes while clone A1L16, a lysozyme specific presenter presented only one of the three epitopes of lysozyme. The latter reveals a hitherto unknown submolecular specificity (to a given epitope within a protein) for antigen presenting cells at the clonal level. Therefore, the specificity of T-cell recognition does not only derive from the T-cell but may also be dependent on the epitope specificity of the antigen-presenting B-cell.

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