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Armstrong PB, Quigley JP. Limulus alpha 2-macroglobulin. First evidence in an invertebrate for a protein containing an internal thiol ester bond. Biochem J. 1987;248(3):703-7doi: 10.1042/bj2480703.
Armstrong, P. B., & Quigley, J. P. (1987). Limulus alpha 2-macroglobulin. First evidence in an invertebrate for a protein containing an internal thiol ester bond. The Biochemical journal, 248(3), 703-7. https://doi.org/10.1042/bj2480703
Armstrong, P B, and Quigley, J P. "Limulus alpha 2-macroglobulin. First evidence in an invertebrate for a protein containing an internal thiol ester bond." The Biochemical journal vol. 248,3 (1987): 703-7. doi: https://doi.org/10.1042/bj2480703
Armstrong PB, Quigley JP. Limulus alpha 2-macroglobulin. First evidence in an invertebrate for a protein containing an internal thiol ester bond. Biochem J. 1987 Dec 15;248(3):703-7. doi: 10.1042/bj2480703. PMID: 2449170; PMCID: PMC1148606.
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Biochem J. 1987 Dec 15;248(3):703-7. doi: 10.1042/bj2480703.

Limulus alpha 2-macroglobulin. First evidence in an invertebrate for a protein containing an internal thiol ester bond.

The Biochemical journal

P B Armstrong, J P Quigley

Affiliations

  1. Marine Biological Laboratory, Woods Hole, MA 02543.

PMID: 2449170 PMCID: PMC1148606 DOI: 10.1042/bj2480703
Free PMC Article

Abstract

Intra-chain thiol ester bonds are present in a limited number of proteins. The thiol ester class of proteins includes vertebrate alpha 2-macroglobulin and the complement proteins C3 and C4. We report here the first instance of a thiol ester protein from an invertebrate, the alpha 2-macroglobulin proteinase-inhibitor homologue present in the plasma of the American horseshoe crab Limulus polyphemus. Our evidence is of three kinds: (1) the proteinase-binding activity of Limulus alpha 2-macroglobulin is inactivated by the low-molecular-mass primary amine methylamine; (2) the native protein is subject to autolytic fragmentation during mild thermal denaturation, yielding fragments of approx. 125 kDa and 55 kDa, whereas the methylamine-treated protein is stable under these conditions of thermal treatment; (3) new thiol groups are generated rapidly during reaction of the protein with trypsin. The demonstration of the thiol ester bond in a protein from an ancient invertebrate provides evolutionary evidence for the importance of this bond in the function of plasma forms of the alpha 2-macroglobulin-like proteinase inhibitors.

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References

  1. Hoppe Seylers Z Physiol Chem. 1982 Jul;363(7):677-82 - PubMed
  2. Anal Biochem. 1976 May 7;72:248-54 - PubMed
  3. Proc Natl Acad Sci U S A. 1980 Dec;77(12):7194-8 - PubMed
  4. J Biol Chem. 1980 Sep 10;255(17):8087-91 - PubMed
  5. Arch Biochem Biophys. 1981 Oct 1;211(1):500-6 - PubMed
  6. J Biol Chem. 1981 Sep 10;256(17):9023-7 - PubMed
  7. Biochem Biophys Res Commun. 1982 Aug 31;107(4):1243-51 - PubMed
  8. FEBS Lett. 1981 Jun 1;128(1):127-32 - PubMed
  9. Proc Natl Acad Sci U S A. 1979 Sep;76(9):4313-6 - PubMed
  10. Springer Semin Immunopathol. 1983;6(4):259-82 - PubMed
  11. Clin Chim Acta. 1966 Oct;14(4):493-501 - PubMed
  12. Methods Enzymol. 1976;45:639-52 - PubMed
  13. Biochemistry. 1980 Sep 16;19(19):4479-85 - PubMed
  14. J Biol Chem. 1983 Jun 25;258(12):7481-9 - PubMed
  15. Arch Biochem Biophys. 1984 Nov 15;235(1):267-75 - PubMed
  16. J Biol Chem. 1983 Dec 10;258(23):14682-5 - PubMed
  17. J Biochem. 1981 Aug;90(2):423-32 - PubMed
  18. Biochem J. 1980 Jul 1;189(1):67-80 - PubMed
  19. J Biol Chem. 1984 Apr 10;259(7):4458-62 - PubMed
  20. Arch Biochem Biophys. 1983 Feb 15;221(1):261-70 - PubMed
  21. Biochem J. 1980 Jun 1;187(3):695-701 - PubMed
  22. J Biol Chem. 1979 Sep 10;254(17):8669-78 - PubMed
  23. J Exp Zool. 1985 Oct;236(1):1-9 - PubMed
  24. Biochem J. 1979 Aug 1;181(2):401-18 - PubMed
  25. J Exp Med. 1970 Nov;132(5):941-50 - PubMed
  26. Biochem J. 1926;20(5):1028-35 - PubMed
  27. J Biol Chem. 1983 Jul 10;258(13):7903-6 - PubMed
  28. Biochem J. 1981 May 1;195(2):453-61 - PubMed
  29. Acta Soc Med Ups. 1961;66:152-70 - PubMed
  30. Biochem J. 1981 May 1;195(2):471-80 - PubMed
  31. J Exp Med. 1980 Oct 1;152(4):1102-14 - PubMed
  32. Biochemistry. 1980 Sep 16;19(19):4471-8 - PubMed
  33. FEBS Lett. 1981 Jun 1;128(1):123-6 - PubMed
  34. J Biol Chem. 1984 Jan 25;259(2):1272-8 - PubMed
  35. J Immunol. 1979 Sep;123(3):1388-94 - PubMed
  36. Ann N Y Acad Sci. 1983;421:1-9 - PubMed
  37. Biochem J. 1985 Oct 15;231(2):417-23 - PubMed
  38. Arch Biochem Biophys. 1961 Nov;95:271-8 - PubMed
  39. Biochemistry. 1984 Jul 3;23(14):3115-24 - PubMed
  40. J Biol Chem. 1979 Jun 10;254(11):4452-6 - PubMed
  41. FEBS Lett. 1980 Dec 1;121(2):275-9 - PubMed
  42. Biochem J. 1973 Aug;133(4):709-24 - PubMed
  43. J Biol Chem. 1981 Jan 25;256(2):547-50 - PubMed
  44. Proc Natl Acad Sci U S A. 1981 Dec;78(12):7388-92 - PubMed
  45. Biochem J. 1984 Jan 1;217(1):303-8 - PubMed
  46. Biochim Biophys Acta. 1980 May 29;623(1):46-56 - PubMed
  47. J Biol Chem. 1980 Nov 10;255(21):10025-8 - PubMed
  48. Exp Cell Res. 1982 Jul;140(1):15-24 - PubMed
  49. J Biol Chem. 1981 Nov 10;256(21):10934-40 - PubMed
  50. Biochemistry. 1981 Apr 28;20(9):2394-402 - PubMed
  51. FEBS Lett. 1985 Mar 11;182(1):125-9 - PubMed
  52. Biochem J. 1983 Jan 1;209(1):99-105 - PubMed
  53. J Biol Chem. 1980 Aug 10;255(15):7082-4 - PubMed
  54. Ann N Y Acad Sci. 1983;421:188-208 - PubMed
  55. Biochem J. 1978 Jul 1;173(1):27-38 - PubMed
  56. Biochem J. 1982 Jul 1;205(1):97-104 - PubMed
  57. J Biochem. 1981 Feb;89(2):659-64 - PubMed
  58. Biochem J. 1981 Jan 1;193(1):115-27 - PubMed
  59. Biochim Biophys Acta. 1968 Jan 22;154(1):228-31 - PubMed
  60. Biochim Biophys Acta. 1985 Mar 1;827(3):453-9 - PubMed
  61. Biochemistry. 1985 May 21;24(11):2653-60 - PubMed
  62. Biochemistry. 1984 Jun 5;23(12):2807-11 - PubMed
  63. Biochem J. 1981 Jan 1;193(1):129-41 - PubMed
  64. J Immunol. 1980 Aug;125(2):634-9 - PubMed
  65. Biochemistry. 1984 Jun 5;23(12):2802-7 - PubMed
  66. J Biol Chem. 1981 Aug 10;256(15):7954-7 - PubMed
  67. Biochemistry. 1982 Aug 31;21(18):4450-8 - PubMed
  68. Scand J Immunol. 1981;13(5):413-31 - PubMed
  69. Proc Natl Acad Sci U S A. 1981 Apr;78(4):2235-9 - PubMed
  70. J Biol Chem. 1985 Oct 15;260(23):12715-9 - PubMed
  71. Biochem J. 1982 Nov 1;207(2):347-56 - PubMed
  72. Ann N Y Acad Sci. 1983;421:259-76 - PubMed
  73. Proc Natl Acad Sci U S A. 1980 Oct;77(10):5764-8 - PubMed
  74. J Biol Chem. 1981 Oct 25;256(20):10409-14 - PubMed
  75. J Exp Med. 1973 Sep 1;138(3):508-21 - PubMed

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