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Báguena-Cervellera R, Renau-Piqueras J, O'Connor JE, et al. Effects of prolonged exposure to ammonia on fluid-phase, receptor-mediated, and adsorptive (non specific) endocytosis in cultured neuroblastoma cells. A flow-cytometry and cytochemical study. Histochemistry. 1987;87(5):445-55doi: 10.1007/BF00496816.
Báguena-Cervellera, R., Renau-Piqueras, J., O'Connor, J. E., & Grisolia, S. (1987). Effects of prolonged exposure to ammonia on fluid-phase, receptor-mediated, and adsorptive (non specific) endocytosis in cultured neuroblastoma cells. A flow-cytometry and cytochemical study. Histochemistry, 87(5), 445-55. https://doi.org/10.1007/BF00496816
Báguena-Cervellera, R, et al. "Effects of prolonged exposure to ammonia on fluid-phase, receptor-mediated, and adsorptive (non specific) endocytosis in cultured neuroblastoma cells. A flow-cytometry and cytochemical study." Histochemistry vol. 87,5 (1987): 445-55. doi: https://doi.org/10.1007/BF00496816
Báguena-Cervellera R, Renau-Piqueras J, O'Connor JE, Grisolia S. Effects of prolonged exposure to ammonia on fluid-phase, receptor-mediated, and adsorptive (non specific) endocytosis in cultured neuroblastoma cells. A flow-cytometry and cytochemical study. Histochemistry. 1987;87(5):445-55. doi: 10.1007/BF00496816. PMID: 2448272.
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Histochemistry. 1987;87(5):445-55. doi: 10.1007/BF00496816.

Effects of prolonged exposure to ammonia on fluid-phase, receptor-mediated, and adsorptive (non specific) endocytosis in cultured neuroblastoma cells. A flow-cytometry and cytochemical study.

Histochemistry

R Báguena-Cervellera, J Renau-Piqueras, J E O'Connor, S Grisolia

Affiliations

  1. Instituto de Investigaciones Citologicas, Valencia, Spain.

PMID: 2448272 DOI: 10.1007/BF00496816

Abstract

The effect of prolonged exposure to ammonia on fluid-phase, receptor-mediated, and adsorptive (non specific) endocytosis in cultured neuroblastoma (Neuro-2a) cells were studied using fluorescein-labeled dextran, concanavalin A conjugated with fluorescein isothiocyanate, and cationized ferritin as tracers. Ammonia treatment increased the rate of endocytosis of cationized ferritin as well as the number of cell elements involved in the process. Moreover, the number of cytoplasmic components containing acid phosphatase activity was also found to increase following ammonia treatment. In contrast, flow-cytometric analyses showed that, under experimental conditions, exposure to ammonia did not alter the intralysosomal pH and had little effect on the fluid-phase and receptor-mediated endocytosis of fluorescein-labeled dextran and concanavalin-A fluorocrome, respectively.

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References

  1. Biol Cell. 1985;54(3):199-205 - PubMed
  2. Histochemistry. 1985;82(3):293-7 - PubMed
  3. J Cell Biol. 1985 Jun;100(6):1894-904 - PubMed
  4. Proc Natl Acad Sci U S A. 1978 Jul;75(7):3327-31 - PubMed
  5. J Cell Biol. 1981 Sep;90(3):656-64 - PubMed
  6. Arch Neurol. 1975 Feb;32(2):123-6 - PubMed
  7. J Cell Biol. 1978 Jun;77(3):R35-42 - PubMed
  8. J Cell Biol. 1982 Nov;95(2 Pt 1):676-81 - PubMed
  9. Brain Res. 1985 Aug 12;340(2):211-8 - PubMed
  10. Methods Enzymol. 1983;98:203-25 - PubMed
  11. Virchows Arch B Cell Pathol Incl Mol Pathol. 1985;49(3):249-59 - PubMed
  12. Biochem Biophys Res Commun. 1984 Mar 15;119(2):516-23 - PubMed
  13. Biochem J. 1984 Jan 1;217(1):27-40 - PubMed
  14. J Cell Biol. 1983 Jan;96(1):277-81 - PubMed
  15. J Histochem Cytochem. 1964 Mar;12:219-21 - PubMed
  16. J Histochem Cytochem. 1983 Oct;31(10):1197-208 - PubMed
  17. J Histochem Cytochem. 1987 Feb;35(2):221-8 - PubMed
  18. Biochem Pharmacol. 1974 Sep 15;23(18):2495-531 - PubMed
  19. J Cell Biol. 1981 Sep;90(3):665-9 - PubMed
  20. J Cell Biol. 1983 Jan;96(1):1-27 - PubMed
  21. Cell Tissue Res. 1985;240(3):743-6 - PubMed
  22. Virchows Arch B Cell Pathol Incl Mol Pathol. 1986;50(3):271-83 - PubMed
  23. Int Rev Cytol. 1984;92:51-92 - PubMed
  24. J Cell Biol. 1980 Jun;85(3):839-52 - PubMed
  25. J Neurol Sci. 1971 Mar;12(3):241-61 - PubMed
  26. J Neuropathol Exp Neurol. 1975 Sep;34(5):425-44 - PubMed
  27. J Cell Biol. 1980 Dec;87(3 Pt 1):579-88 - PubMed
  28. J Cell Biol. 1983 Jan;96(1):51-62 - PubMed
  29. J Histochem Cytochem. 1985 Aug;33(8):749-54 - PubMed
  30. Acta Neurol Scand Suppl. 1978;71:1-144 - PubMed

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