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Buergisser DM, Roth BV, Luethi C, et al. Expression of a human insulin-like growth factor II cDNA in NIH-3T3 cells. Biochem Biophys Res Commun. 1990;169(3):832-9doi: 10.1016/0006-291x(90)91968-x.
Buergisser, D. M., Roth, B. V., Luethi, C., Gerber, H. P., Honegger, A., & Humbel, R. E. (1990). Expression of a human insulin-like growth factor II cDNA in NIH-3T3 cells. Biochemical and biophysical research communications, 169(3), 832-9. https://doi.org/10.1016/0006-291x(90)91968-x
Buergisser, D M, et al. "Expression of a human insulin-like growth factor II cDNA in NIH-3T3 cells." Biochemical and biophysical research communications vol. 169,3 (1990): 832-9. doi: https://doi.org/10.1016/0006-291x(90)91968-x
Buergisser DM, Roth BV, Luethi C, Gerber HP, Honegger A, Humbel RE. Expression of a human insulin-like growth factor II cDNA in NIH-3T3 cells. Biochem Biophys Res Commun. 1990 Jun 29;169(3):832-9. doi: 10.1016/0006-291x(90)91968-x. PMID: 2363726.
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Biochem Biophys Res Commun. 1990 Jun 29;169(3):832-9. doi: 10.1016/0006-291x(90)91968-x.

Expression of a human insulin-like growth factor II cDNA in NIH-3T3 cells.

Biochemical and biophysical research communications

D M Buergisser, B V Roth, C Luethi, H P Gerber, A Honegger, R E Humbel

Affiliations

  1. Department of Biochemistry, University of Zürich, Switzerland.

PMID: 2363726 DOI: 10.1016/0006-291x(90)91968-x

Abstract

Recombinant human insulin-like growth factor II (IGF-II) was produced in NIH-3T3 cells transfected with a plasmid containing a construct encoding the signal peptide and the sequence of mature human IGF II. Successfully transfected clones secreted correctly processed recombinant human IGF II at rates of about 100ng per 24 hours per 10(6) cells. The biological activity of the purified recombinant human IGF II exhibited similar potencies as standard human IGF II isolated from serum in radio-receptorassays as well as in thymidine incorporation assays.

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