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McKenzie B, Kay G, Matthews KH, et al. The hen's egg chorioallantoic membrane (HET-CAM) test to predict the ophthalmic irritation potential of a cysteamine-containing gel: Quantification using Photoshop® and ImageJ. Int J Pharm. 2015;490(1):1-8doi: 10.1016/j.ijpharm.2015.05.023.
McKenzie, B., Kay, G., Matthews, K. H., Knott, R. M., & Cairns, D. (2015). The hen's egg chorioallantoic membrane (HET-CAM) test to predict the ophthalmic irritation potential of a cysteamine-containing gel: Quantification using Photoshop® and ImageJ. International journal of pharmaceutics, 490(1), 1-8. https://doi.org/10.1016/j.ijpharm.2015.05.023
McKenzie, Barbara, et al. "The hen's egg chorioallantoic membrane (HET-CAM) test to predict the ophthalmic irritation potential of a cysteamine-containing gel: Quantification using Photoshop® and ImageJ." International journal of pharmaceutics vol. 490,1 (2015): 1-8. doi: https://doi.org/10.1016/j.ijpharm.2015.05.023
McKenzie B, Kay G, Matthews KH, Knott RM, Cairns D. The hen's egg chorioallantoic membrane (HET-CAM) test to predict the ophthalmic irritation potential of a cysteamine-containing gel: Quantification using Photoshop® and ImageJ. Int J Pharm. 2015 Jul 25;490(1):1-8. doi: 10.1016/j.ijpharm.2015.05.023. Epub 2015 May 14. PMID: 25980731.
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Int J Pharm. 2015 Jul 25;490(1):1-8. doi: 10.1016/j.ijpharm.2015.05.023. Epub 2015 May 14.

The hen's egg chorioallantoic membrane (HET-CAM) test to predict the ophthalmic irritation potential of a cysteamine-containing gel: Quantification using Photoshop® and ImageJ.

International journal of pharmaceutics

Barbara McKenzie, Graeme Kay, Kerr H Matthews, Rachel M Knott, Donald Cairns

Affiliations

  1. Institute of Health & Wellbeing Research, Robert Gordon University, Aberdeen, AB10 7GJ, UK. Electronic address: [email protected].
  2. Institute of Health & Wellbeing Research, Robert Gordon University, Aberdeen, AB10 7GJ, UK.
  3. School of Pharmacy and Life Sciences, Robert Gordon University, Aberdeen, AB10 7GJ, UK.

PMID: 25980731 DOI: 10.1016/j.ijpharm.2015.05.023

Abstract

A modified hen's egg chorioallantoic membrane (HET-CAM) test has been developed, combining ImageJ analysis with Adobe(®) Photoshop(®). The irritation potential of an ophthalmic medicine can be quantified using this method, by monitoring damage to blood vessels. The evaluation of cysteamine containing hyaluronate gel is reported. The results demonstrated that the novel gel formulation is non-irritant to the ocular tissues, in line with saline solution (negative control). In conclusion, the modification of the established HET-CAM test can quantify the damage to minute blood vessels. These results offer the possibility to formulate cysteamine in an ocular applicable gel formulation.

Copyright © 2015 Elsevier B.V. All rights reserved.

Keywords: Cysteamine; Cystinosis; HET-CAM test; ImageJ; Ophthalmic; Photoshop(®)

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