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El-Badry OM, Romanus JA, Helman LJ, et al. Autonomous growth of a human neuroblastoma cell line is mediated by insulin-like growth factor II. J Clin Invest. 1989;84(3):829-39doi: 10.1172/JCI114243.
El-Badry, O. M., Romanus, J. A., Helman, L. J., Cooper, M. J., Rechler, M. M., & Israel, M. A. (1989). Autonomous growth of a human neuroblastoma cell line is mediated by insulin-like growth factor II. The Journal of clinical investigation, 84(3), 829-39. https://doi.org/10.1172/JCI114243
El-Badry, O M, et al. "Autonomous growth of a human neuroblastoma cell line is mediated by insulin-like growth factor II." The Journal of clinical investigation vol. 84,3 (1989): 829-39. doi: https://doi.org/10.1172/JCI114243
El-Badry OM, Romanus JA, Helman LJ, Cooper MJ, Rechler MM, Israel MA. Autonomous growth of a human neuroblastoma cell line is mediated by insulin-like growth factor II. J Clin Invest. 1989 Sep;84(3):829-39. doi: 10.1172/JCI114243. PMID: 2547840; PMCID: PMC329726.
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American Society for Clinical Investigation Free PMC Article

J Clin Invest. 1989 Sep;84(3):829-39. doi: 10.1172/JCI114243.

Autonomous growth of a human neuroblastoma cell line is mediated by insulin-like growth factor II.

The Journal of clinical investigation

O M El-Badry, J A Romanus, L J Helman, M J Cooper, M M Rechler, M A Israel

Affiliations

  1. Molecular Genetics Section, National Cancer Institute, Bethesda, Maryland 20892.

PMID: 2547840 PMCID: PMC329726 DOI: 10.1172/JCI114243
Free PMC Article

Abstract

Insulin-like growth factor II (IGF-II) mRNA was increased in two of eight neuroblastomas and in eight of eight pheochromocytomas, tumors of the adrenal medulla that occur in childhood and adulthood, respectively. RNA encoding the type I IGF receptor, the receptor thought to mediate the mitogenic effects of IGF-I and IGF-II, also was uniformly expressed in these cells. To assess the role of IGF-II in the growth of these tumor cells, we have used the SK-N-AS cultured neuroblastoma cell line, which can be continuously propagated in mitogen-free medium, as a model system. Our results strongly suggest that IGF-II, synthesized by SK-N-AS cells and acting through type I IGF receptors, contributes to the autonomous growth of this tumor cell line. (a) SK-N-AS cells synthesized large amounts of IGF-II RNA and secreted greater than 50 ng/ml of IGF-II (as determined by specific radioimmuno- and radioreceptor assays). Little, if any, IGF-I RNA or immunoreactive IGF-I were detected. (b) SK-N-AS cells possess type I IGF receptors. (c) Exogenous IGF-I and IGF-II stimulated DNA synthesis in SK-N-AS cells, and this stimulation was abolished by a blocking antibody to the type I IGF receptor. (d) This anti-receptor antibody also abolished the multiplication of SK-N-AS cells in the absence of added mitogens. We conclude that IGF-II is an autocrine growth factor for SK-N-AS cells and suggest that this mechanism may contribute to the growth of some adrenal medullary tumors.

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