Display options
Cite
Alam J, Baek KJ, Choi YS, et al. N-acetylcysteine and the human serum components that inhibit bacterial invasion of gingival epithelial cells prevent experimental periodontitis in mice. J Periodontal Implant Sci. 2014;44(6):266-73doi: 10.5051/jpis.2014.44.6.266.
Alam, J., Baek, K. J., Choi, Y. S., Kim, Y. C., & Choi, Y. (2014). N-acetylcysteine and the human serum components that inhibit bacterial invasion of gingival epithelial cells prevent experimental periodontitis in mice. Journal of periodontal & implant science, 44(6), 266-73. https://doi.org/10.5051/jpis.2014.44.6.266
Alam, Jehan, et al. "N-acetylcysteine and the human serum components that inhibit bacterial invasion of gingival epithelial cells prevent experimental periodontitis in mice." Journal of periodontal & implant science vol. 44,6 (2014): 266-73. doi: https://doi.org/10.5051/jpis.2014.44.6.266
Alam J, Baek KJ, Choi YS, Kim YC, Choi Y. N-acetylcysteine and the human serum components that inhibit bacterial invasion of gingival epithelial cells prevent experimental periodontitis in mice. J Periodontal Implant Sci. 2014 Dec;44(6):266-73. doi: 10.5051/jpis.2014.44.6.266. Epub 2014 Dec 31. PMID: 25568806; PMCID: PMC4284374.
Copy Download .nbib Format: NLM
Share it on

J Periodontal Implant Sci. 2014 Dec;44(6):266-73. doi: 10.5051/jpis.2014.44.6.266. Epub 2014 Dec 31.

N-acetylcysteine and the human serum components that inhibit bacterial invasion of gingival epithelial cells prevent experimental periodontitis in mice.

Journal of periodontal & implant science

Jehan Alam, Keum Jin Baek, Yun Sik Choi, Yong Cheol Kim, Youngnim Choi

Affiliations

  1. Department of Oral Microbiology and Immunology, Dental Research Institute, Seoul National University School of Dentistry, Seoul, Korea.

PMID: 25568806 PMCID: PMC4284374 DOI: 10.5051/jpis.2014.44.6.266

Abstract

PURPOSE: We previously reported that human serum significantly reduces the invasion of various oral bacterial species into gingival epithelial cells in vitro. The aims of the present study were to characterize the serum component(s) responsible for the inhibition of bacterial invasion of epithelial cells and to examine their effect on periodontitis induced in mice.

METHODS: Immortalized human gingival epithelial (HOK-16B) cells were infected with various 5- (and 6-) carboxy-fluorescein diacetate succinimidyl ester-labeled oral bacteria, including Fusobacterium nucleatum, Provetella intermedia, Porphyromonas gingivalis, and Treponiema denticola, in the absence or presence of three major serum components (human serum albumin [HSA], pooled human IgG [phIgG] and α1-antitrypsin). Bacterial adhesion and invasion were determined by flow cytometry. The levels of intracellular reactive oxygen species (ROS) and activation of small GTPases were examined. Experimental periodontitis was induced by oral inoculation of P. gingivalis and T. denticola in Balb/c mice.

RESULTS: HSA and phIgG, but not α1-antitrypsin, efficiently inhibited the invasion of various oral bacterial species into HOK-16B cells. HSA but not phIgG decreased the adhesion of F. nucleatum onto host cells and the levels of intracellular ROS in HOK-16B cells. N-acetylcysteine (NAC), a ROS scavenger, decreased both the levels of intracellular ROS and invasion of F. nucleatum into HOK-16B cells, confirming the role of ROS in bacterial invasion. Infection with F. nucleatum activated Rac1, a regulator of actin cytoskeleton dynamics. Not only HSA and NAC but also phIgG decreased the F. nucleatum-induced activation of Rac1. Furthermore, both HSA plus phIgG and NAC significantly reduced the alveolar bone loss in the experimental periodontitis induced by P. gingivalis and T. denticola in mice.

CONCLUSIONS: NAC and the serum components HSA and phIgG, which inhibit bacterial invasion of oral epithelial cells in vitro, can successfully prevent experimental periodontitis.

Keywords: Albumins; Bacteria; Epithelial cells; Immunoglobulin G; Periodontitis

References

  1. Free Radic Biol Med. 2009 Nov 1;47(9):1239-53 - PubMed
  2. Infect Immun. 1990 Apr;58(4):1017-25 - PubMed
  3. Periodontol 2000. 2002;30:61-9 - PubMed
  4. Mol Biol Cell. 2004 Mar;15(3):1003-10 - PubMed
  5. J Periodontal Res. 2010 Apr;45(2):229-38 - PubMed
  6. Cell. 1992 Aug 7;70(3):401-10 - PubMed
  7. Genes Dev. 1997 Sep 15;11(18):2295-322 - PubMed
  8. Infect Immun. 2009 Jul;77(7):3075-9 - PubMed
  9. Ann Intensive Care. 2013 Feb 15;3(1):4 - PubMed
  10. J Periodontol. 2014 Aug;85(8):e313-22 - PubMed
  11. Cell Microbiol. 2007 Oct;9(10):2431-44 - PubMed
  12. PLoS One. 2013;8(1):e53703 - PubMed
  13. Periodontol 2000. 2004;35:53-74 - PubMed
  14. J Clin Periodontol. 2001 Mar;28(3):264-9 - PubMed
  15. Arch Oral Biol. 2013 Oct;58(10):1282-92 - PubMed
  16. Microbiology. 2008 Oct;154(Pt 10):3122-33 - PubMed
  17. J Biomater Appl. 1991 Jan;5(3):204-26 - PubMed
  18. Methods Mol Biol. 2008;425:27-39 - PubMed
  19. J Periodontal Res. 2012 Apr;47(2):248-62 - PubMed
  20. J Clin Periodontol. 1998 Feb;25(2):134-44 - PubMed
  21. J Cell Sci. 2001 Apr;114(Pt 7):1331-41 - PubMed
  22. FEMS Microbiol Lett. 2007 Jul;272(1):8-14 - PubMed
  23. Eur Cell Mater. 2004 Dec 07;8:37-57 - PubMed
  24. Infect Immun. 1997 May;65(5):1980-4 - PubMed
  25. Mol Cells. 2010 Dec;30(6):519-26 - PubMed
  26. Cell Microbiol. 2002 May;4(5):305-14 - PubMed
  27. J Periodontal Res. 2015 Oct;50(5):570-85 - PubMed
  28. J Periodontol. 2009 Apr;80(4):672-8 - PubMed
  29. PLoS One. 2012;7(8):e41342 - PubMed
  30. Periodontol 2000. 2002;30:70-8 - PubMed
  31. Arch Oral Biol. 1994 Dec;39(12):1035-40 - PubMed
  32. Immunol Cell Biol. 2012 Jul;90(6):655-63 - PubMed

Publication Types