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Zeidel ML, Kikeri D, Silva P, et al. Atrial natriuretic peptides inhibit conductive sodium uptake by rabbit inner medullary collecting duct cells. J Clin Invest. 1988;82(3):1067-74doi: 10.1172/JCI113663.
Zeidel, M. L., Kikeri, D., Silva, P., Burrowes, M., & Brenner, B. M. (1988). Atrial natriuretic peptides inhibit conductive sodium uptake by rabbit inner medullary collecting duct cells. The Journal of clinical investigation, 82(3), 1067-74. https://doi.org/10.1172/JCI113663
Zeidel, M L, et al. "Atrial natriuretic peptides inhibit conductive sodium uptake by rabbit inner medullary collecting duct cells." The Journal of clinical investigation vol. 82,3 (1988): 1067-74. doi: https://doi.org/10.1172/JCI113663
Zeidel ML, Kikeri D, Silva P, Burrowes M, Brenner BM. Atrial natriuretic peptides inhibit conductive sodium uptake by rabbit inner medullary collecting duct cells. J Clin Invest. 1988 Sep;82(3):1067-74. doi: 10.1172/JCI113663. PMID: 2458385; PMCID: PMC303621.
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American Society for Clinical Investigation Free PMC Article

J Clin Invest. 1988 Sep;82(3):1067-74. doi: 10.1172/JCI113663.

Atrial natriuretic peptides inhibit conductive sodium uptake by rabbit inner medullary collecting duct cells.

The Journal of clinical investigation

M L Zeidel, D Kikeri, P Silva, M Burrowes, B M Brenner

Affiliations

  1. Renal Division, Brigham and Women's Hospital, Boston, Massachusetts 02115.

PMID: 2458385 PMCID: PMC303621 DOI: 10.1172/JCI113663
Free PMC Article

Abstract

The inner medullary collecting duct (IMCD) effects net sodium reabsorption under the control of volume regulatory hormones, including atrial natriuretic peptides (ANP). These studies examined the mechanisms of sodium transport and its regulation by ANP in fresh suspensions of IMCD cells. Sodium uptake was inhibited by amiloride but insensitive to furosemide, bu-metanide, and hydrochlorthiazide. These results are consistent with uptake mediated by a sodium channel or Na+/H+ exchange. To determine the role of sodium channels, cells were hyperpolarized by preincubation in high potassium medium followed by dilution into potassium-free medium. Membrane potential measurements using the cyanine dye, Di(S)-C3-5 verified a striking hyperpolarization of IMCD cells using this protocol. Hyperpolarization increased the apparent initial rate of sodium uptake fourfold. Amiloride and ANP inhibited potential-stimulated sodium uptake 73% and 65%, respectively; the two agents together were not additive. Addition of 5 mM sodium to hyperpolarized cells resulted in a significant amiloride-sensitive depolarization. Half-maximal inhibition of potential-driven sodium uptake occurred at 3 X 10(-7) M amiloride, and 5 X 10(-11) M ANP. We conclude that sodium enters IMCD cells via a conductive, amiloride-sensitive sodium channel, which is regulated by ANP. ANP inhibition of luminal sodium entry in the IMCD appears to contribute to the marked natriuretic effect of this hormone in vivo.

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