Display options
Cite
Zhu D, Ravindranath MH, Terasaki PI, et al. Suppression of allo-human leucocyte antigen (HLA) antibodies secreted by B memory cells in vitro: intravenous immunoglobulin (IVIg) versus a monoclonal anti-HLA-E IgG that mimics HLA-I reactivities of IVIg. Clin Exp Immunol. 2014;177(2):464-77doi: 10.1111/cei.12307.
Zhu, D., Ravindranath, M. H., Terasaki, P. I., Miyazaki, T., Pham, T., & Jucaud, V. (2014). Suppression of allo-human leucocyte antigen (HLA) antibodies secreted by B memory cells in vitro: intravenous immunoglobulin (IVIg) versus a monoclonal anti-HLA-E IgG that mimics HLA-I reactivities of IVIg. Clinical and experimental immunology, 177(2), 464-77. https://doi.org/10.1111/cei.12307
Zhu, D, et al. "Suppression of allo-human leucocyte antigen (HLA) antibodies secreted by B memory cells in vitro: intravenous immunoglobulin (IVIg) versus a monoclonal anti-HLA-E IgG that mimics HLA-I reactivities of IVIg." Clinical and experimental immunology vol. 177,2 (2014): 464-77. doi: https://doi.org/10.1111/cei.12307
Zhu D, Ravindranath MH, Terasaki PI, Miyazaki T, Pham T, Jucaud V. Suppression of allo-human leucocyte antigen (HLA) antibodies secreted by B memory cells in vitro: intravenous immunoglobulin (IVIg) versus a monoclonal anti-HLA-E IgG that mimics HLA-I reactivities of IVIg. Clin Exp Immunol. 2014 Aug;177(2):464-77. doi: 10.1111/cei.12307. PMID: 24611451; PMCID: PMC4226597.
Copy Download .nbib Format: NLM
Share it on

Clin Exp Immunol. 2014 Aug;177(2):464-77. doi: 10.1111/cei.12307.

Suppression of allo-human leucocyte antigen (HLA) antibodies secreted by B memory cells in vitro: intravenous immunoglobulin (IVIg) versus a monoclonal anti-HLA-E IgG that mimics HLA-I reactivities of IVIg.

Clinical and experimental immunology

D Zhu, M H Ravindranath, P I Terasaki, T Miyazaki, T Pham, V Jucaud

Affiliations

  1. Terasaki Foundation Laboratory, Los Angeles, CA, USA; Department of Urology, Zhongshan Hospital, Fudan University, Shanghai, China.

PMID: 24611451 PMCID: PMC4226597 DOI: 10.1111/cei.12307

Abstract

B memory cells remain in circulation and secrete alloantibodies without antigen exposure > 20 years after alloimmunization postpartum or by transplantation. These long-lived B cells are resistant to cytostatic drugs. Therapeutically, intravenous immunoglobulin (IVIg) is administered to reduce allo-human leucocyte antigen (HLA) antibodies pre- and post-transplantation, but the mechanism of reduction remains unclear. Recently, we reported that IVIg reacts with several HLA-I alleles and the HLA reactivity of IVIg is lost after its HLA-E reactivity is adsorbed out. Therefore, we have generated an anti-HLA-E monoclonal antibody that mimics the HLA-reactivity of IVIg to investigate whether this antibody suppresses IgG secretion, as does IVIg. B cells were purified from the blood of a woman in whose blood the B memory cells remained without antigen exposure > 20 years after postpartum alloimmunization. The B cells were stimulated with cytokines using a well-defined culture system. The anti-HLA-E monoclonal antibody (mAb) significantly suppressed the allo-HLA class-II IgG produced by the B cells, and that this suppression was far superior to that by IVIg. These findings were confirmed with HLA-I antibody secreted by the immortalized B cell line, developed from the blood of another alloimmunized woman. The binding affinity of the anti-HLA-E mAb for peptide sequences shared (i.e. shared epitopes) between HLA-E and other β2-microglobulin-free HLA heavy chains (open conformers) on the cell surface of B cells may act as a ligand and signal suppression of IgG production of activated B memory cells. We propose that anti-HLA-E monoclonal antibody may also be useful to suppress allo-HLA IgG production in vivo.

© 2014 British Society for Immunology.

Keywords: B memory cells; HLA-reactivity; allo-HLA IgG; immunosuppression; intravenous immunoglobulin (IVIg)

References

  1. Blood. 2011 Jul 28;118(4):992-1001 - PubMed
  2. N Engl J Med. 1991 Jul 11;325(2):110-7 - PubMed
  3. Pediatr Transplant. 2005 Jun;9(3):408-15 - PubMed
  4. Blood. 2007 Apr 1;109(7):2806-14 - PubMed
  5. J Immunol. 2005 Mar 15;174(6):3773-80 - PubMed
  6. Transplantation. 1994 Feb 27;57(4):553-62 - PubMed
  7. Blood. 2003 Apr 15;101(8):3065-73 - PubMed
  8. Clin J Am Soc Nephrol. 2011 Apr;6(4):922-36 - PubMed
  9. Science. 1996 Apr 5;272(5258):54-60 - PubMed
  10. Clin Exp Immunol. 1991 Nov;86(2):192-8 - PubMed
  11. Immunol Rev. 1994 Jun;139:125-58 - PubMed
  12. Blood. 2002 May 1;99(9):3256-62 - PubMed
  13. Transplantation. 2012 Aug 27;94(4):345-51 - PubMed
  14. J Immunol. 2005 Dec 15;175(12):7867-79 - PubMed
  15. N Engl J Med. 2008 Jul 17;359(3):242-51 - PubMed
  16. Clin Exp Immunol. 2009 Oct;158(1):99-105 - PubMed
  17. Nat Rev Immunol. 2006 Oct;6(10):741-50 - PubMed
  18. N Engl J Med. 2011 Jul 28;365(4):318-26 - PubMed
  19. Immunol Cell Biol. 1997 Oct;75(5):446-55 - PubMed
  20. Transplantation. 2012 Jul 27;94(2):165-71 - PubMed
  21. J Immunol. 1998 Oct 1;161(7):3781-90 - PubMed
  22. Hum Immunol. 2009 Jan;70(1):29-34 - PubMed
  23. Am J Transplant. 2002 Sep;2(8):758-60 - PubMed
  24. J Allergy Clin Immunol. 2001 Oct;108(4 Suppl):S95-8 - PubMed
  25. Transplantation. 2008 Aug 15;86(3):377-83 - PubMed
  26. J Autoimmun. 1991 Apr;4(2):325-39 - PubMed
  27. Blood. 2009 Dec 10;114(25):5173-81 - PubMed
  28. Transplantation. 2003 May 15;75(9):1490-5 - PubMed
  29. J Exp Med. 2010 Jan 18;207(1):155-71 - PubMed
  30. J Immunol. 2003 Nov 1;171(9):4621-9 - PubMed
  31. Pediatr Allergy Immunol. 1994 May;5(2):63-87 - PubMed
  32. Boll Soc Ital Biol Sper. 1990 Dec;66(12):1193-200 - PubMed
  33. Transplantation. 2003 Aug 27;76(4):631-6 - PubMed
  34. J Autoimmun. 2011 Feb;36(1):9-15 - PubMed
  35. Allerg Immunol (Paris). 1993 Jan;25(1):35-7 - PubMed
  36. J Clin Immunol. 1991 May;11(3):152-8 - PubMed
  37. Transplantation. 1996 Sep 15;62(5):691-3 - PubMed
  38. Clin Immunol Immunopathol. 1994 Nov;73(2):180-6 - PubMed
  39. Mol Immunol. 2011 Jan;48(4):423-30 - PubMed
  40. Int Immunol. 1998 Nov;10(11):1703-11 - PubMed
  41. Mol Immunol. 2010 Feb;47(5):1121-31 - PubMed
  42. Am J Transplant. 2007 Apr;7(4):864-71 - PubMed
  43. Trends Immunol. 2007 Mar;28(3):115-23 - PubMed
  44. Blood. 2013 Mar 14;121(11):2013-28 - PubMed
  45. Curr Opin Immunol. 2005 Oct;17(5):541-5 - PubMed
  46. Transplantation. 1993 Aug;56(2):335-7 - PubMed
  47. J Immunol. 2009 Feb 1;182(3):1548-59 - PubMed
  48. Blood. 2001 Mar 15;97(6):1817-22 - PubMed
  49. J Immunol. 2010 Aug 1;185(3):1935-48 - PubMed
  50. Clin Transplant. 2012 May-Jun;26(3):E261-8 - PubMed
  51. J Allergy Clin Immunol. 2009 Oct;124(4):819-26.e4 - PubMed

Substances

MeSH terms

Publication Types