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Salipante SJ, Hoogestraat DR, Abbott AN, et al. Coinfection of Fusobacterium nucleatum and Actinomyces israelii in mastoiditis diagnosed by next-generation DNA sequencing. J Clin Microbiol. 2014;52(5):1789-92doi: 10.1128/JCM.03133-13.
Salipante, S. J., Hoogestraat, D. R., Abbott, A. N., SenGupta, D. J., Cummings, L. A., Butler-Wu, S. M., Stephens, K., Cookson, B. T., & Hoffman, N. G. (2014). Coinfection of Fusobacterium nucleatum and Actinomyces israelii in mastoiditis diagnosed by next-generation DNA sequencing. Journal of clinical microbiology, 52(5), 1789-92. https://doi.org/10.1128/JCM.03133-13
Salipante, Stephen J, et al. "Coinfection of Fusobacterium nucleatum and Actinomyces israelii in mastoiditis diagnosed by next-generation DNA sequencing." Journal of clinical microbiology vol. 52,5 (2014): 1789-92. doi: https://doi.org/10.1128/JCM.03133-13
Salipante SJ, Hoogestraat DR, Abbott AN, SenGupta DJ, Cummings LA, Butler-Wu SM, Stephens K, Cookson BT, Hoffman NG. Coinfection of Fusobacterium nucleatum and Actinomyces israelii in mastoiditis diagnosed by next-generation DNA sequencing. J Clin Microbiol. 2014 May;52(5):1789-92. doi: 10.1128/JCM.03133-13. Epub 2014 Feb 26. PMID: 24574281; PMCID: PMC3993667.
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J Clin Microbiol. 2014 May;52(5):1789-92. doi: 10.1128/JCM.03133-13. Epub 2014 Feb 26.

Coinfection of Fusobacterium nucleatum and Actinomyces israelii in mastoiditis diagnosed by next-generation DNA sequencing.

Journal of clinical microbiology

Stephen J Salipante, Daniel R Hoogestraat, April N Abbott, Dhruba J SenGupta, Lisa A Cummings, Susan M Butler-Wu, Karen Stephens, Brad T Cookson, Noah G Hoffman

Affiliations

  1. Department of Laboratory Medicine, University of Washington, Seattle, Washington, USA.

PMID: 24574281 PMCID: PMC3993667 DOI: 10.1128/JCM.03133-13

Abstract

Some bacterial infections involve potentially complex mixtures of species that can now be distinguished using next-generation DNA sequencing. We present a case of mastoiditis where Gram stain, culture, and molecular diagnosis were nondiagnostic or discrepant. Next-generation sequencing implicated coinfection of Fusobacterium nucleatum and Actinomyces israelii, resolving these diagnostic discrepancies.

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