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Guo X, Wang A, Li X, et al. Studies on monoclonal antibody against recombinant human granulocyte colony-stimulating factor. Chin Med Sci J. 1991;6(4):212-6
Guo, X., Wang, A., Li, X., Guo, Z., & Zhang, Y. (1991). Studies on monoclonal antibody against recombinant human granulocyte colony-stimulating factor. Chinese medical sciences journal = Chung-kuo i hsueh k'o hsueh tsa chih, 6(4), 212-6.
Guo, X, et al. "Studies on monoclonal antibody against recombinant human granulocyte colony-stimulating factor." Chinese medical sciences journal = Chung-kuo i hsueh k'o hsueh tsa chih vol. 6,4 (1991): 212-6.
Guo X, Wang A, Li X, Guo Z, Zhang Y. Studies on monoclonal antibody against recombinant human granulocyte colony-stimulating factor. Chin Med Sci J. 1991 Dec;6(4):212-6. PMID: 1725968.
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Chin Med Sci J. 1991 Dec;6(4):212-6.

Studies on monoclonal antibody against recombinant human granulocyte colony-stimulating factor.

Chinese medical sciences journal = Chung-kuo i hsueh k'o hsueh tsa chih

X Guo, A Wang, X Li, Z Guo, Y Zhang

Affiliations

  1. PUMC Hospital, CAMS, Beijing.

PMID: 1725968

Abstract

Three hybridoma cell lines producing monoclonal antibodies (McAb) against recombinant human granulocyte colony-stimulating factor (rhG-CSF) have been established by fusing mouse myeloma cell line SP2/0 cells with spleen cells from a BALB/c mouse immunized with rhG-CSF. Ascites was obtained from BALB/c mice by injecting the hybridoma cells intraperitoneally. Three McAbs were purified by the caprylic acid-ammonium sulfate method. For each, the IgG subclass, valence and activity, molecular weight, specificity and affinity were determined. The applications of McAbs against rhG-CSF in the clinic and laboratory are discussed.

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