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Watson R, Lindner S, Bordereau P, et al. Standardisation of the factor H autoantibody assay. Immunobiology. 2013;219(1):9-16doi: 10.1016/j.imbio.2013.06.004.
Watson, R., Lindner, S., Bordereau, P., Hunze, E. M., Tak, F., Ngo, S., Zipfel, P. F., Skerka, C., Dragon-Durey, M. A., & Marchbank, K. J. (2014). Standardisation of the factor H autoantibody assay. Immunobiology, 219(1), 9-16. https://doi.org/10.1016/j.imbio.2013.06.004
Watson, Rachael, et al. "Standardisation of the factor H autoantibody assay." Immunobiology vol. 219,1 (2014): 9-16. doi: https://doi.org/10.1016/j.imbio.2013.06.004
Watson R, Lindner S, Bordereau P, Hunze EM, Tak F, Ngo S, Zipfel PF, Skerka C, Dragon-Durey MA, Marchbank KJ. Standardisation of the factor H autoantibody assay. Immunobiology. 2014 Jan;219(1):9-16. doi: 10.1016/j.imbio.2013.06.004. Epub 2013 Jun 26. PMID: 23891327.
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Immunobiology. 2014 Jan;219(1):9-16. doi: 10.1016/j.imbio.2013.06.004. Epub 2013 Jun 26.

Standardisation of the factor H autoantibody assay.

Immunobiology

Rachael Watson, Susanne Lindner, Pauline Bordereau, Eva-Maria Hunze, Federico Tak, Stéphanie Ngo, Peter F Zipfel, Christine Skerka, Marie-Agnes Dragon-Durey, Kevin J Marchbank

Affiliations

  1. Institute of Cellular Medicine, Newcastle University, Newcastle-upon-Tyne, UK.

PMID: 23891327 DOI: 10.1016/j.imbio.2013.06.004

Abstract

The screening of all atypical haemolytic uraemic syndrome (aHUS) patients for factor H autoantibodies is best practice. However, there is no consensus assay for the reporting of factor H autoantibody titres. In this study, three European complement laboratories with expertise in the field of autoantibody testing address this by systematically evaluating several ELISA methods used for the detection of factor H autoantibodies. All methods tested adequately detect high titre samples. However, this study recommends the Paris method for the detection and reporting of factor H autoantibodies to be used when setting up a factor H autoantibody screen. The importance of individual sample background subtraction in these ELISA tests was established. The use of a relative or arbitrary unit index with a common positive and negative serum allowed for consistent comparison of findings from different test centres. Therefore, it is recommended that a standard arbitrary unit scale based on a titration curve from a common positive anti-serum be adopted to allow future establishment of the relative importance of particular titres of factor H autoantibodies in aHUS. Systematic assay for the presence of factor H autoantibodies in patients using the Paris method will provide the longitudinal analysis needed to fully establish the importance of factor H autoantibodies in disease. This will feed into additional research to clarify whether additional factors have a bearing on the phenotype/outcome of autoimmune aHUS.

Copyright © 2013 Elsevier GmbH. All rights reserved.

Keywords: AI-aHUS; AU; Autoantibody; Complement; ECGRD; ELISA; European Working Party on Complement Genetics in Renal Disease; Factor H; HRPO; IC; IgG; Kidney disease; SCR; aHUS; arbitrary units; atypical haemolytic uraemic syndrome; autoimmune atypical haemolytic uraemic syndrome; enzyme linked immunosorbent assay; horse-radish peroxidase; immune complex; immunoglobulin G; short consensus repeat

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