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Macchia E, Nakai A, Janiga A, et al. Characterization of site-specific polyclonal antibodies to c-erbA peptides recognizing human thyroid hormone receptors alpha 1, alpha 2, and beta and native 3,5,3'-triiodothyronine receptor, and study of tissue distribution of the antigen. Endocrinology. 1990;126(6):3232-9doi: 10.1210/endo-126-6-3232.
Macchia, E., Nakai, A., Janiga, A., Sakurai, A., Fisfalen, M. E., Gardner, P., Soltani, K., & DeGroot, L. J. (1990). Characterization of site-specific polyclonal antibodies to c-erbA peptides recognizing human thyroid hormone receptors alpha 1, alpha 2, and beta and native 3,5,3'-triiodothyronine receptor, and study of tissue distribution of the antigen. Endocrinology, 126(6), 3232-9. https://doi.org/10.1210/endo-126-6-3232
Macchia, E, et al. "Characterization of site-specific polyclonal antibodies to c-erbA peptides recognizing human thyroid hormone receptors alpha 1, alpha 2, and beta and native 3,5,3'-triiodothyronine receptor, and study of tissue distribution of the antigen." Endocrinology vol. 126,6 (1990): 3232-9. doi: https://doi.org/10.1210/endo-126-6-3232
Macchia E, Nakai A, Janiga A, Sakurai A, Fisfalen ME, Gardner P, Soltani K, DeGroot LJ. Characterization of site-specific polyclonal antibodies to c-erbA peptides recognizing human thyroid hormone receptors alpha 1, alpha 2, and beta and native 3,5,3'-triiodothyronine receptor, and study of tissue distribution of the antigen. Endocrinology. 1990 Jun;126(6):3232-9. doi: 10.1210/endo-126-6-3232. PMID: 1693571.
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Endocrinology. 1990 Jun;126(6):3232-9. doi: 10.1210/endo-126-6-3232.

Characterization of site-specific polyclonal antibodies to c-erbA peptides recognizing human thyroid hormone receptors alpha 1, alpha 2, and beta and native 3,5,3'-triiodothyronine receptor, and study of tissue distribution of the antigen.

Endocrinology

E Macchia, A Nakai, A Janiga, A Sakurai, M E Fisfalen, P Gardner, K Soltani, L J DeGroot

Affiliations

  1. Department of Medicine, University of Chicago, Illinois 60637.

PMID: 1693571 DOI: 10.1210/endo-126-6-3232

Abstract

The translated products of v-erbA-related cDNAs have been demonstrated to be thyroid hormone receptors, and three different forms of receptor (alpha 1, alpha 2, and beta) have been found in human tissues. We synthesized five peptides corresponding to different portions of these three receptors and raised site-specific polyclonal-antipeptide sera in rabbits. Each antibody displayed high titer and specificity for its respective antigen when tested in an enzyme-linked immunosorbent assay. Each immunoprecipitated the corresponding in vitro translated products of human c-erbA alpha 1, alpha 2, or beta. Two of the antisera were specific for beta, one for alpha 2, and one detected a sequence common to alpha 1 and alpha 2. The fifth was directed toward the DNA-binding area of the proteins and interacted with each receptor. The four antibodies against alpha 1 and beta immunoprecipitated the native thyroid hormone receptor from rat liver and caused a partial shift in the elution profile of the native receptor labeled with [125I]T3 on Sephacryl S-300 column chromatography. The antibody against alpha 2 protein did not interact with native thyroid hormone receptor from rat liver. Using the indirect immunofluorescence technique with the five antibodies, we detected immunoreactivity primarily in the nucleus of cells in several tissues. In general, there was coordinate expression of both alpha and beta receptors in each organ examined, in agreement with previous data on tissue distribution of mRNAs for human thyroid hormone receptors. These studies prove the identity of v-erbA-related gene products with native thyroid hormone receptors and the expression of both alpha and beta receptors in nuclei of human and rat tissues.

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