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Miyake S, Sugiyama H, Tani Y, et al. Identification of a recombinational signal sequence-specific DNA-binding protein(s) of Mr 115,000 in the nuclear extracts from immature lymphoid cell lines. J Immunogenet. 1990;17(1):67-75doi: 10.1111/j.1744-313x.1990.tb00860.x.
Miyake, S., Sugiyama, H., Tani, Y., Fukuda, T., & Kishimoto, S. (1990). Identification of a recombinational signal sequence-specific DNA-binding protein(s) of Mr 115,000 in the nuclear extracts from immature lymphoid cell lines. Journal of immunogenetics, 17(1), 67-75. https://doi.org/10.1111/j.1744-313x.1990.tb00860.x
Miyake, S, et al. "Identification of a recombinational signal sequence-specific DNA-binding protein(s) of Mr 115,000 in the nuclear extracts from immature lymphoid cell lines." Journal of immunogenetics vol. 17,1 (1990): 67-75. doi: https://doi.org/10.1111/j.1744-313x.1990.tb00860.x
Miyake S, Sugiyama H, Tani Y, Fukuda T, Kishimoto S. Identification of a recombinational signal sequence-specific DNA-binding protein(s) of Mr 115,000 in the nuclear extracts from immature lymphoid cell lines. J Immunogenet. 1990 Feb-Apr;17(1):67-75. doi: 10.1111/j.1744-313x.1990.tb00860.x. PMID: 2212701.
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J Immunogenet. 1990 Feb-Apr;17(1):67-75. doi: 10.1111/j.1744-313x.1990.tb00860.x.

Identification of a recombinational signal sequence-specific DNA-binding protein(s) of Mr 115,000 in the nuclear extracts from immature lymphoid cell lines.

Journal of immunogenetics

S Miyake, H Sugiyama, Y Tani, T Fukuda, S Kishimoto

Affiliations

  1. Osaka University Medical School, Japan.

PMID: 2212701 DOI: 10.1111/j.1744-313x.1990.tb00860.x

Abstract

Rearrangements of immunoglobulin genes are mediated by highly conserved heptamer and nonamer recombinational signal sequence. Using a protein-blotting procedure, a heptamer and nonamer recombinational signal sequence-specific DNA-binding protein(s) was examined in the nuclear extracts from lymphoid and nonlymphoid cell lines. Nuclear extracts were subjected to SDS-polyacrylamide gel electrophoresis, and transferred by electroblotting to nitrocellulose filters. Then the filters were hybridized to 32P-labelled synthetic double-stranded heptamer-23bp-nonamer or nonamer-12bp-heptamer recombinational signal sequence probes. A relatively large amount of a DNA-binding protein(s) of Mr 115,000 for both probes was detected in the nuclear extracts from immature B and immature T cell lines. No DNA-binding proteins were detected in a myeloma cell line. Interestingly, this DNA-binding protein(s) might be able to recognize both heptamer and nonamer. Recombinational signal sequence-specific DNA-binding activity of the protein(s) and the presence of the protein(s) in a stage-specific manner strongly suggest that the protein(s) of Mr 115,000 detected here may play an important role in the recombination of Ig and TCR genes.

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