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Nigam S, Nodes S, Cichon G, et al. Receptor-mediated action of hepoxilin A3 releases diacylglycerol and arachidonic acid from human neutrophils. Biochem Biophys Res Commun. 1990;171(3):944-8doi: 10.1016/0006-291x(90)90775-i.
Nigam, S., Nodes, S., Cichon, G., Corey, E. J., & Pace-Asciak, C. R. (1990). Receptor-mediated action of hepoxilin A3 releases diacylglycerol and arachidonic acid from human neutrophils. Biochemical and biophysical research communications, 171(3), 944-8. https://doi.org/10.1016/0006-291x(90)90775-i
Nigam, S, et al. "Receptor-mediated action of hepoxilin A3 releases diacylglycerol and arachidonic acid from human neutrophils." Biochemical and biophysical research communications vol. 171,3 (1990): 944-8. doi: https://doi.org/10.1016/0006-291x(90)90775-i
Nigam S, Nodes S, Cichon G, Corey EJ, Pace-Asciak CR. Receptor-mediated action of hepoxilin A3 releases diacylglycerol and arachidonic acid from human neutrophils. Biochem Biophys Res Commun. 1990 Sep 28;171(3):944-8. doi: 10.1016/0006-291x(90)90775-i. PMID: 2171511.
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Biochem Biophys Res Commun. 1990 Sep 28;171(3):944-8. doi: 10.1016/0006-291x(90)90775-i.

Receptor-mediated action of hepoxilin A3 releases diacylglycerol and arachidonic acid from human neutrophils.

Biochemical and biophysical research communications

S Nigam, S Nodes, G Cichon, E J Corey, C R Pace-Asciak

Affiliations

  1. Department of Gynaecological Endocrinology, Klinikum Steglitz, Free University of Berlin, FRG.

PMID: 2171511 DOI: 10.1016/0006-291x(90)90775-i

Abstract

We have previously shown that hepoxilin A3 increases the intracellular concentration of Ca+2 in human neutrophils. Herein we address the initial events of hepoxilin action on the neutrophil which precede the rise in intracellular calcium. We show that hepoxilin A3 at 10-1000 nM concentrations releases from [1-14C]-arachidonic acid labeled neutrophils diacylglycerol and unesterified arachidonic acid in a time and concentration dependent fashion. The release of arachidonic acid and diacyglycerol are receptor-mediated events which are blocked by pertussis toxin. This data shows that hepoxilin A3 stimulates phospholipases C and A2 in the cell which may be involved in the rise in cytosolic calcium. Thus, hepoxilins may represent a hitherto unrecognised class of cellular mediators.

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