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Thomas JA, Weintraub ST, Wu W, et al. Extensive proteolysis of head and inner body proteins by a morphogenetic protease in the giant Pseudomonas aeruginosa phage φKZ. Mol Microbiol. 2012;84(2):324-39doi: 10.1111/j.1365-2958.2012.08025.x.
Thomas, J. A., Weintraub, S. T., Wu, W., Winkler, D. C., Cheng, N., Steven, A. C., & Black, L. W. (2012). Extensive proteolysis of head and inner body proteins by a morphogenetic protease in the giant Pseudomonas aeruginosa phage φKZ. Molecular microbiology, 84(2), 324-39. https://doi.org/10.1111/j.1365-2958.2012.08025.x
Thomas, Julie A, et al. "Extensive proteolysis of head and inner body proteins by a morphogenetic protease in the giant Pseudomonas aeruginosa phage φKZ." Molecular microbiology vol. 84,2 (2012): 324-39. doi: https://doi.org/10.1111/j.1365-2958.2012.08025.x
Thomas JA, Weintraub ST, Wu W, Winkler DC, Cheng N, Steven AC, Black LW. Extensive proteolysis of head and inner body proteins by a morphogenetic protease in the giant Pseudomonas aeruginosa phage φKZ. Mol Microbiol. 2012 Apr;84(2):324-39. doi: 10.1111/j.1365-2958.2012.08025.x. Epub 2012 Mar 20. PMID: 22429790; PMCID: PMC3323674.
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Mol Microbiol. 2012 Apr;84(2):324-39. doi: 10.1111/j.1365-2958.2012.08025.x. Epub 2012 Mar 20.

Extensive proteolysis of head and inner body proteins by a morphogenetic protease in the giant Pseudomonas aeruginosa phage φKZ.

Molecular microbiology

Julie A Thomas, Susan T Weintraub, Weimin Wu, Dennis C Winkler, Naiqian Cheng, Alasdair C Steven, Lindsay W Black

Affiliations

  1. Biochemistry and Molecular Biology, University of Maryland Baltimore, MD 21201, USA.

PMID: 22429790 PMCID: PMC3323674 DOI: 10.1111/j.1365-2958.2012.08025.x

Abstract

Encased within the 280 kb genome in the capsid of the giant myovirus φKZ is an unusual cylindrical proteinaceous 'inner body' of highly ordered structure. We present here mass spectrometry, bioinformatic and biochemical studies that reveal novel information about the φKZ head and the complex inner body. The identification of 39 cleavage sites in 19 φKZ head proteins indicates cleavage of many prohead proteins forms a major morphogenetic step in φKZ head maturation. The φKZ head protease, gp175, is newly identified here by a bioinformatics approach, as confirmed by a protein expression assay. Gp175 is distantly related to T4 gp21 and recognizes and cleaves head precursors at related but distinct S/A/G-X-E recognition sites. Within the φKZ head there are six high-copy-number proteins that are probable major components of the inner body. The molecular weights of five of these proteins are reduced 35-65% by cleavages making their mature form similar (26-31 kDa), while their precursors are dissimilar (36-88 kDa). Together the six abundant proteins sum to the estimated mass of the inner body (15-20 MDa). The identification of these proteins is important for future studies on the composition and function of the inner body.

© 2012 Blackwell Publishing Ltd.

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