Display options
Cite
Cephas KD, Kim J, Mathai RA, et al. Comparative analysis of salivary bacterial microbiome diversity in edentulous infants and their mothers or primary care givers using pyrosequencing. PLoS One. 2011;6(8):e23503doi: 10.1371/journal.pone.0023503.
Cephas, K. D., Kim, J., Mathai, R. A., Barry, K. A., Dowd, S. E., Meline, B. S., & Swanson, K. S. (2011). Comparative analysis of salivary bacterial microbiome diversity in edentulous infants and their mothers or primary care givers using pyrosequencing. PloS one, 6(8), e23503. https://doi.org/10.1371/journal.pone.0023503
Cephas, Kimberly D, et al. "Comparative analysis of salivary bacterial microbiome diversity in edentulous infants and their mothers or primary care givers using pyrosequencing." PloS one vol. 6,8 (2011): e23503. doi: https://doi.org/10.1371/journal.pone.0023503
Cephas KD, Kim J, Mathai RA, Barry KA, Dowd SE, Meline BS, Swanson KS. Comparative analysis of salivary bacterial microbiome diversity in edentulous infants and their mothers or primary care givers using pyrosequencing. PLoS One. 2011;6(8):e23503. doi: 10.1371/journal.pone.0023503. Epub 2011 Aug 10. PMID: 21853142; PMCID: PMC3154475.
Copy Download .nbib Format: NLM
Share it on

PLoS One. 2011;6(8):e23503. doi: 10.1371/journal.pone.0023503. Epub 2011 Aug 10.

Comparative analysis of salivary bacterial microbiome diversity in edentulous infants and their mothers or primary care givers using pyrosequencing.

PloS one

Kimberly D Cephas, Juhee Kim, Rose Ann Mathai, Kathleen A Barry, Scot E Dowd, Brandon S Meline, Kelly S Swanson

Affiliations

  1. Division of Nutritional Sciences, University of Illinois, Urbana, Illinois, United States of America.

PMID: 21853142 PMCID: PMC3154475 DOI: 10.1371/journal.pone.0023503

Abstract

Bacterial contribution to oral disease has been studied in young children, but there is a lack of data addressing the developmental perspective in edentulous infants. Our primary objectives were to use pyrosequencing to phylogenetically characterize the salivary bacterial microbiome of edentulous infants and to make comparisons against their mothers. Saliva samples were collected from 5 edentulous infants (mean age = 4.6±1.2 mo old) and their mothers or primary care givers (mean age = 30.8±9.5 y old). Salivary DNA was extracted, used to generate DNA amplicons of the V4-V6 hypervariable region of the bacterial 16S rDNA gene, and subjected to 454-pyrosequencing. On average, over 80,000 sequences per sample were generated. High bacterial diversity was noted in the saliva of adults [1012 operational taxonomical units (OTU) at 3% divergence] and infants (578 OTU at 3% divergence). Firmicutes, Proteobacteria, Actinobacteria, and Fusobacteria were predominant bacterial phyla present in all samples. A total of 397 bacterial genera were present in our dataset. Of the 28 genera different (P<0.05) between infants and adults, 27 had a greater prevalence in adults. The exception was Streptococcus, which was the predominant genera in infant saliva (62.2% in infants vs. 20.4% in adults; P<0.05). Veillonella, Neisseria, Rothia, Haemophilus, Gemella, Granulicatella, Leptotrichia, and Fusobacterium were also predominant genera in infant samples, while Haemophilus, Neisseria, Veillonella, Fusobacterium, Oribacterium, Rothia, Treponema, and Actinomyces were predominant in adults. Our data demonstrate that although the adult saliva bacterial microbiome had a greater OTU count than infants, a rich bacterial community exists in the infant oral cavity prior to tooth eruption. Streptococcus, Veillonella, and Neisseria are the predominant bacterial genera present in infants. Further research is required to characterize the development of oral microbiota early in life and identify environmental factors that impact colonization and oral and gastrointestinal disease risk.

References

  1. J Periodontal Res. 2004 Aug;39(4):213-20 - PubMed
  2. J Clin Microbiol. 2011 Apr;49(4):1464-74 - PubMed
  3. Appl Environ Microbiol. 2009 Dec;75(23):7537-41 - PubMed
  4. PLoS Pathog. 2010 Jan 08;6(1):e1000713 - PubMed
  5. Ann Periodontol. 2002 Dec;7(1):8-16 - PubMed
  6. Open Microbiol J. 2010 Aug 11;4:47-52 - PubMed
  7. Microb Ecol. 2010 Oct;60(3):677-90 - PubMed
  8. J Microbiol Methods. 2009 Dec;79(3):266-71 - PubMed
  9. J Dent Res. 1993 Jan;72(1):37-45 - PubMed
  10. J Periodontol. 2009 Sep;80(9):1421-32 - PubMed
  11. Foodborne Pathog Dis. 2008 Aug;5(4):459-72 - PubMed
  12. Nat Methods. 2010 May;7(5):335-6 - PubMed
  13. Oral Microbiol Immunol. 1994 Jun;9(3):136-41 - PubMed
  14. Appl Environ Microbiol. 2005 Mar;71(3):1501-6 - PubMed
  15. J Clin Periodontol. 1998 Feb;25(2):134-44 - PubMed
  16. Biotechniques. 2004 May;36(5):808-12 - PubMed
  17. Arch Oral Biol. 2003 May;48(5):329-36 - PubMed
  18. J Dent Res. 2008 Nov;87(11):1016-20 - PubMed
  19. Proc Natl Acad Sci U S A. 2010 Jun 29;107(26):11971-5 - PubMed
  20. J Clin Microbiol. 2008 Apr;46(4):1407-17 - PubMed
  21. Int J Paediatr Dent. 2009 May;19(3):186-92 - PubMed
  22. Brain Behav Immun. 2011 Mar;25(3):397-407 - PubMed
  23. Nat Rev Microbiol. 2010 Jul;8(7):471-80 - PubMed
  24. Open Microbiol J. 2010 Mar 17;4:8-19 - PubMed
  25. Int J Paediatr Dent. 2009 May;19(3):193-200 - PubMed
  26. BMC Med Genomics. 2010 Sep 21;3:41 - PubMed
  27. Oral Microbiol Immunol. 1992 Feb;7(1):28-31 - PubMed
  28. BMC Microbiol. 2009 Dec 15;9:259 - PubMed
  29. Microbiol Rev. 1986 Dec;50(4):353-80 - PubMed
  30. Caries Res. 2009;43(4):308-13 - PubMed
  31. Genome Res. 2009 Apr;19(4):636-43 - PubMed
  32. Anal Biochem. 2009 Aug 1;391(1):64-8 - PubMed
  33. Nucleic Acids Res. 1997 Dec 15;25(24):4876-82 - PubMed
  34. BMC Microbiol. 2009 Oct 02;9:210 - PubMed
  35. Nucleic Acids Res. 2004 Mar 19;32(5):1792-7 - PubMed
  36. Caries Res. 2010;44(5):485-97 - PubMed
  37. MMWR Surveill Summ. 2005 Aug 26;54(3):1-43 - PubMed

MeSH terms

Publication Types