Zhonghua Nan Ke Xue. 2011 Jun;17(6):516-22.

[Expression of annexin A7 in spermatogonial stem cells].

Zhonghua nan ke xue = National journal of andrology

[Article in Chinese]
Chun-Mei Yu, Ping Zhang, Jing Wang, Ming-Xi Liu, Hui Wang, Zuo-Min Zhou, Jia-Hao Sha

PMID: 21735650

Abstract

OBJECTIVE: To study the expression of Annexin A7 in the mouse testis, especially in different types of spermatogonia.

METHODS: We prepared Annexin A7 recombinant protein using prokaryotic expression, adsorbed the Annexin A7 antibody with it after identified by mass spectrometry, and detected the expression of Annexin A7 by Western-blot and immunohistochemistry.

RESULTS: Annexin A7 was expressed in a development-dependent manner in the spermatogonia of the prepubertal mice and in the type-A single (As) and type-A paired (Apr) spermatogonia of adult mice. These results were confirmed by the co-localization of Annexin A7 and Stra8, a known determinant of differentiated spermatogonial stem cells (SSCs).

CONCLUSION: Annexin A7 is the internal factor of As and Apr spermatogonia, which might be involved in the biological functions of SSCs.

Substances

• Annexin A7

MeSH terms

• Animals
• Annexin A7 / metabolism
• Male
• Mice
• Spermatogonia / cytology
• Spermatogonia / metabolism
• Stem Cells / cytology
• Stem Cells / metabolism

Publication Types

• English Abstract
• Journal Article