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Estrela CR, Pimenta FC, Alencar AH, et al. Detection of selected bacterial species in intraoral sites of patients with chronic periodontitis using multiplex polymerase chain reaction. J Appl Oral Sci. 2010;18(4):426-31doi: 10.1590/s1678-77572010000400018.
Estrela, C. R., Pimenta, F. C., Alencar, A. H., Ruiz, L. F., & Estrela, C. (2010). Detection of selected bacterial species in intraoral sites of patients with chronic periodontitis using multiplex polymerase chain reaction. Journal of applied oral science : revista FOB, 18(4), 426-31. https://doi.org/10.1590/s1678-77572010000400018
Estrela, Cyntia Rodrigues de Araújo, et al. "Detection of selected bacterial species in intraoral sites of patients with chronic periodontitis using multiplex polymerase chain reaction." Journal of applied oral science : revista FOB vol. 18,4 (2010): 426-31. doi: https://doi.org/10.1590/s1678-77572010000400018
Estrela CR, Pimenta FC, Alencar AH, Ruiz LF, Estrela C. Detection of selected bacterial species in intraoral sites of patients with chronic periodontitis using multiplex polymerase chain reaction. J Appl Oral Sci. 2010 Jul-Aug;18(4):426-31. doi: 10.1590/s1678-77572010000400018. PMID: 20835581; PMCID: PMC5349072.
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J Appl Oral Sci. 2010 Jul-Aug;18(4):426-31. doi: 10.1590/s1678-77572010000400018.

Detection of selected bacterial species in intraoral sites of patients with chronic periodontitis using multiplex polymerase chain reaction.

Journal of applied oral science : revista FOB

Cyntia Rodrigues de Araújo Estrela, Fabiana Cristina Pimenta, Ana Helena Gonçalves de Alencar, Luis Fernando Naldi Ruiz, Carlos Estrela

Affiliations

  1. Institute of Biological Sciences, Federal University of Goiás, Goiânia, GO, Brazil.

PMID: 20835581 PMCID: PMC5349072 DOI: 10.1590/s1678-77572010000400018

Abstract

OBJECTIVE: The aim of this study was to detect the prevalence of selected bacterial species in intraoral sites of patients with chronic periodontitis (CP) using multiplex polymerase chain reaction (PCR).

METHODOLOGY: Samples were collected from the tongue dorsum, buccal mucosa, supragingival and subgingival plaque and saliva of 30 patients with untreated CP. Multiplex PCR was used to determine prevalence rates, which were then compared using a chi-square test. Significance level was set at p<0.05. Mean and standard deviation values were used to evaluate variations in prevalence according to site.

RESULTS: The prevalence of S. mutans was 70% in saliva; 60% in samples collected from the tongue dorsum; 50% in samples collected from the buccal mucosa; 56.5% in the supragingival plaque; and 53.5% in the subgingival plaque. The prevalence of E. faecalis ranged from 3.5% to 13.5% in all intraoral microenvironment. The highest prevalence of P. gingivalis was found in subgingival plaque (53.5%), and of P. intermedia in supragingival plaque (33.5%), subgingival plaque (30%) and tongue dorsum (33.5%). The prevalence of bacteria did not vary significantly among the intraoral sites.

CONCLUSIONS: All studied bacteria were identified in intraoral sites. S. mutans, P. gingivalis and P. intermedia had high prevalence rates, but the prevalence of E. faecalis was low. Multiplex PCR proved to be an adequate method for epidemiological studies.

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