Appl Opt. 2009 Dec 01;48(34):H113-9. doi: 10.1364/AO.48.00H113.

Three-dimensional microscopy and sectional image reconstruction using optical scanning holography.

Applied optics

Edmund Y Lam, Xin Zhang, Huy Vo, Ting-Chung Poon, Guy Indebetouw

PMID: 19956281 DOI: 10.1364/AO.48.00H113

Abstract

Fast acquisition and high axial resolution are two primary requirements for three-dimensional microscopy. However, they are sometimes conflicting: imaging modalities such as confocal imaging can deliver superior resolution at the expense of sequential acquisition at different axial planes, which is a time-consuming process. Optical scanning holography (OSH) promises to deliver a good trade-off between these two goals. With just a single scan, we can capture the entire three-dimensional volume in a digital hologram; the data can then be processed to obtain the individual sections. An accurate modeling of the imaging system is key to devising an appropriate image reconstruction algorithm, especially for real data where random noise and other imaging imperfections must be taken into account. In this paper we demonstrate sectional image reconstruction by applying an inverse imaging sectioning technique to experimental OSH data of biological specimens and visualizing the sections using the OSA Interactive Science Publishing software.

MeSH terms

• Computer-Aided Design
• Equipment Design
• Equipment Failure Analysis
• Holography / instrumentation
• Image Enhancement / instrumentation
• Image Interpretation, Computer-Assisted / instrumentation
• Imaging, Three-Dimensional / instrumentation
• Microscopy, Confocal / instrumentation

Publication Types

• Journal Article
• Research Support, Non-U.S. Gov't