Display options
Cite
Rudolph G, Schilbach-Stückle K, Handgretinger R, et al. Cytogenetic and molecular characterization of a newly established neuroblastoma cell line LS. Hum Genet. 1991;86(6):562-6doi: 10.1007/BF00201542.
Rudolph, G., Schilbach-Stückle, K., Handgretinger, R., Kaiser, P., & Hameister, H. (1991). Cytogenetic and molecular characterization of a newly established neuroblastoma cell line LS. Human genetics, 86(6), 562-6. https://doi.org/10.1007/BF00201542
Rudolph, G, et al. "Cytogenetic and molecular characterization of a newly established neuroblastoma cell line LS." Human genetics vol. 86,6 (1991): 562-6. doi: https://doi.org/10.1007/BF00201542
Rudolph G, Schilbach-Stückle K, Handgretinger R, Kaiser P, Hameister H. Cytogenetic and molecular characterization of a newly established neuroblastoma cell line LS. Hum Genet. 1991 Apr;86(6):562-6. doi: 10.1007/BF00201542. PMID: 2026421.
Copy Download .nbib Format: NLM
Share it on

Hum Genet. 1991 Apr;86(6):562-6. doi: 10.1007/BF00201542.

Cytogenetic and molecular characterization of a newly established neuroblastoma cell line LS.

Human genetics

G Rudolph, K Schilbach-Stückle, R Handgretinger, P Kaiser, H Hameister

Affiliations

  1. Abteilung Klinische Genetik, Universität, Tübingen, Federal Republic of Germany.

PMID: 2026421 DOI: 10.1007/BF00201542

Abstract

A new human neuroblastoma cell line (LS) that originated from an abdominal tumor of a 16-month-old girl is presented; it was classified, according to Evans, as being stage III. Morphological (dense-core particles) and biochemical characteristics (dopamine-beta-hydroxylase, acetylcholinesterase, neuron-specific-enolase) confirmed the diagnosis. In addition to a slightly variable modal chromosome number of 48 or 49 (because of marker-chromosomes and autosomal trisomies), cytogenetic analysis revealed two constantly appearing chromosomes with homogeneously stained regions (HSR's). The karyotype remained constant over 50 passages in vitro [49,XX, -12, +der5, + 17, + mar1, + mar2]. Double minutes were a rare phenomenon and appeared only in a few metaphases. In situ hybridization showed that some of the HSR's consisted of amplified N-myc copies. The distribution of the N-myc copies according to in situ hybridization signals along the HSR's was compared with the data of Southern and Northern blotting analyses.

Cited by

References

  1. Science. 1984 Jun 8;224(4653):1121-4 - PubMed
  2. Cancer Genet Cytogenet. 1982 May;6(1):17-28 - PubMed
  3. Nature. 1984 Mar 15-21;308(5956):288-91 - PubMed
  4. Adv Morphog. 1970;8:41-114 - PubMed
  5. Cancer Genet Cytogenet. 1988 Mar;31(1):83-94 - PubMed
  6. Cancer. 1989 May 1;63(9):1752-6 - PubMed
  7. Proc Natl Acad Sci U S A. 1988 Jul;85(13):4804-8 - PubMed
  8. Science. 1984 Dec 7;226(4679):1199-204 - PubMed
  9. Cancer Res. 1989 May 15;49(10):2683-8 - PubMed
  10. Cancer Res. 1977 May;37(5):1364-71 - PubMed
  11. Proc Natl Acad Sci U S A. 1983 Mar;80(6):1707-11 - PubMed
  12. Surgery. 1990 Jan;107(1):43-9 - PubMed
  13. Cell. 1983 Dec;35(2 Pt 1):359-67 - PubMed
  14. Proc Natl Acad Sci U S A. 1984 Aug;81(15):4940-4 - PubMed
  15. Proc Natl Acad Sci U S A. 1986 Mar;83(6):1772-6 - PubMed
  16. Cancer Res. 1981 Nov;41(11 Pt 1):4678-86 - PubMed
  17. Pediatrics. 1969 Mar;43(3):377-82 - PubMed
  18. Proc Natl Acad Sci U S A. 1983 Sep;80(17):5330-4 - PubMed
  19. Nature. 1985 Jul 11-17;316(6024):160-2 - PubMed
  20. Cancer. 1990 Jun 1;65(11):2572-5 - PubMed
  21. Chromosoma. 1981;83(3):431-9 - PubMed
  22. Cancer Res. 1986 Jun;46(6):3196-9 - PubMed
  23. Arch Dis Child. 1981 Apr;56(4):271-4 - PubMed
  24. Proc Natl Acad Sci U S A. 1977 Dec;74(12):5729-33 - PubMed
  25. Science. 1984 Dec 14;226(4680):1335-7 - PubMed
  26. Nature. 1987 Aug 6-12;328(6130):524-6 - PubMed
  27. Cancer. 1971 Feb;27(2):374-8 - PubMed
  28. Proc Natl Acad Sci U S A. 1985 Jun;82(11):3736-40 - PubMed
  29. Cancer Genet Cytogenet. 1982 Feb;5(1):81-94 - PubMed
  30. N Engl J Med. 1980 May 29;302(22):1254-6 - PubMed
  31. Science. 1977 Nov 18;198(4318):739-41 - PubMed
  32. Nature. 1983 Aug 18-24;304(5927):596-602 - PubMed
  33. N Engl J Med. 1984 Jul 26;311(4):231-5 - PubMed
  34. Histopathology. 1990 May;16(5):461-7 - PubMed
  35. Hum Genet. 1980;54(2):277-8 - PubMed
  36. Gynakologe. 1984 Mar;17(1):33-40 - PubMed
  37. Cancer. 1977 Nov;40(5):2256-63 - PubMed
  38. Cancer. 1972 Jun;29(6):1671-9 - PubMed
  39. Cancer Res. 1987 Jan 1;47(1):311-8 - PubMed
  40. Exp Cell Res. 1977 Mar 15;105(2):301-11 - PubMed
  41. Cancer Res. 1984 Nov;44(11):5444-9 - PubMed
  42. Nature. 1986 Jan 2-8;319(6048):73-7 - PubMed
  43. Nature. 1989 Mar 23;338(6213):348-50 - PubMed
  44. Br J Cancer. 1988 Sep;58(3):287-91 - PubMed
  45. Int J Cancer. 1983 Jun 15;31(6):765-8 - PubMed
  46. Int J Cancer. 1979 Mar 15;23(3):299-301 - PubMed
  47. Nucleic Acids Res. 1987 Jun 25;15(12):4761-70 - PubMed
  48. Cancer. 1980 Mar 1;45(5):833-9 - PubMed
  49. Nature. 1983 Sep 15-21;305(5931):245-8 - PubMed

Substances

MeSH terms

Publication Types

LinkOut - more resources