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In Vitro Cell Dev Biol. 1991 Feb;27(2):142-6. doi: 10.1007/BF02631000.

Reconstruction of the skin in three-dimensional collagen gel matrix culture.

In vitro cellular & developmental biology : journal of the Tissue Culture Association

H Sugihara, S Toda, S Miyabara, Y Kusaba, Y Minami

Affiliations

  1. Department of Pathology, Saga Medical School, Japan.

PMID: 2019554 DOI: 10.1007/BF02631000

Abstract

The skin comprises three layers: epidermis, dermis, and hypodermis. We report here on a skin, reconstructed in vitro, that is composed of all three layers. The top-most layer, epidermis, was exposed to air by a new method. The exposure induced an extensive proliferation, and differentiation, i.e. keratinization was eventually observed in the cultured epidermal cells. Skin thus cultured will be a useful graft of transplantation and provide an ideal model system in which to study diseases of the skin.

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