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Stoeckle MY. Post-transcriptional regulation of gro alpha, beta, gamma, and IL-8 mRNAs by IL-1 beta. Nucleic Acids Res. 1991;19(4):917-20doi: 10.1093/nar/19.4.917.
Stoeckle, M. Y. (1991). Post-transcriptional regulation of gro alpha, beta, gamma, and IL-8 mRNAs by IL-1 beta. Nucleic acids research, 19(4), 917-20. https://doi.org/10.1093/nar/19.4.917
Stoeckle, M Y. "Post-transcriptional regulation of gro alpha, beta, gamma, and IL-8 mRNAs by IL-1 beta." Nucleic acids research vol. 19,4 (1991): 917-20. doi: https://doi.org/10.1093/nar/19.4.917
Stoeckle MY. Post-transcriptional regulation of gro alpha, beta, gamma, and IL-8 mRNAs by IL-1 beta. Nucleic Acids Res. 1991 Feb 25;19(4):917-20. doi: 10.1093/nar/19.4.917. PMID: 2017372; PMCID: PMC333731.
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Nucleic Acids Res. 1991 Feb 25;19(4):917-20. doi: 10.1093/nar/19.4.917.

Post-transcriptional regulation of gro alpha, beta, gamma, and IL-8 mRNAs by IL-1 beta.

Nucleic acids research

M Y Stoeckle

Affiliations

  1. Division of Infectious Diseases, Cornell University Medical College, New York, NY 10021.

PMID: 2017372 PMCID: PMC333731 DOI: 10.1093/nar/19.4.917
Free PMC Article

Abstract

Expression of the cytokine gene gro, also known as melanoma growth stimulatory activity, is induced by inflammatory stimuli, including IL-1. To determine whether gro expression is regulated at a post-transcriptional level, the effect of IL-1 on gro mRNA stability was examined. Treatment of fibroblasts with IL-1 beta caused a dose-dependent induction of gro mRNA. When IL-1 was withdrawn, gro mRNA decayed rapidly with a half life of 1 hour. This decay occurred whether or not actinomycin D was added to block new transcription. In contrast, when IL-1 was present in the medium, the level of gro mRNA was stable over 8 hours following addition of actinomycin D. In addition, the stability of a related mRNA, IL-8, was found to be regulated by IL-1. To examine whether Northern results reflected expression of gro alpha, or of the closely related genes, gro beta and gro gamma, RNA samples were analyzed by PCR. All three genes were found to be induced by IL-1 and all mRNAs were stabilized in the presence of IL-1. Northern analysis revealed a minor species of gro mRNA which lacked poly(A). The pattern of expression of this RNA suggested that it was a decay intermediate of one or more of the gro mRNAs. The findings indicate that mRNA stabilization is an important component of IL-1 induced gene expression.

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References

  1. Nature. 1988 Aug 18;334(6183):567-8 - PubMed
  2. New Biol. 1989 Nov;1(2):121-6 - PubMed
  3. Nature. 1988 Nov 24;336(6197):396-9 - PubMed
  4. Proc Natl Acad Sci U S A. 1988 Dec;85(24):9645-9 - PubMed
  5. Cell. 1988 Dec 23;55(6):1115-22 - PubMed
  6. Genes Dev. 1989 Jan;3(1):60-72 - PubMed
  7. Science. 1989 Apr 21;244(4902):339-43 - PubMed
  8. Biochem J. 1989 Apr 15;259(2):585-8 - PubMed
  9. EMBO J. 1989 Jun;8(6):1761-6 - PubMed
  10. FASEB J. 1989 Dec;3(14):2565-73 - PubMed
  11. Mol Cell Biol. 1989 Nov;9(11):4738-45 - PubMed
  12. J Immunol. 1990 Mar 15;144(6):2223-32 - PubMed
  13. J Exp Med. 1990 Apr 1;171(4):1091-100 - PubMed
  14. J Exp Med. 1990 May 1;171(5):1797-802 - PubMed
  15. J Exp Med. 1990 Sep 1;172(3):911-9 - PubMed
  16. Mol Cell Biol. 1990 Oct;10(10):5596-9 - PubMed
  17. Nucleic Acids Res. 1985 Apr 11;13(7):2485-502 - PubMed
  18. Cell. 1986 Aug 29;46(5):659-67 - PubMed
  19. Cell. 1987 Feb 27;48(4):671-9 - PubMed
  20. Proc Natl Acad Sci U S A. 1987 Oct;84(20):7188-92 - PubMed
  21. J Cell Biochem. 1988 Feb;36(2):185-98 - PubMed
  22. J Exp Med. 1988 Jun 1;167(6):1883-93 - PubMed
  23. Proc Natl Acad Sci U S A. 1990 Oct;87(19):7732-6 - PubMed
  24. New Biol. 1990 Apr;2(4):313-23 - PubMed
  25. EMBO J. 1988 Jul;7(7):2025-33 - PubMed

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