Display options
Share it on

Appl Environ Microbiol. 2008 Mar;74(6):1757-66. doi: 10.1128/AEM.02740-07. Epub 2008 Feb 01.

Human Salmonella clinical isolates distinct from those of animal origin.

Applied and environmental microbiology

Douglas M Heithoff, William R Shimp, Patrick W Lau, Golnaz Badie, Elena Y Enioutina, Raymond A Daynes, Barbara A Byrne, John K House, Michael J Mahan

Affiliations

  1. Department of Molecular, Cellular and Developmental Biology, University of California, Santa Barbara, CA 93106, USA.

PMID: 18245251 PMCID: PMC2268321 DOI: 10.1128/AEM.02740-07

Abstract

The global trend toward intensive livestock production has led to significant public health risks and industry-associated losses due to an increased incidence of disease and contamination of livestock-derived food products. A potential factor contributing to these health concerns is the prospect that selective pressure within a particular host may give rise to bacterial strain variants that exhibit enhanced fitness in the present host relative to that in the parental host from which the strain was derived. Here, we assessed 184 Salmonella enterica human and animal clinical isolates for their virulence capacities in mice and for the presence of the Salmonella virulence plasmid encoding the SpvB actin cytotoxin required for systemic survival and Pef fimbriae, implicated in adherence to the murine intestinal epithelium. All (21 of 21) serovar Typhimurium clinical isolates derived from animals were virulent in mice, whereas many (16 of 41) serovar Typhimurium isolates derived from human salmonellosis patients lacked this capacity. Additionally, many (10 of 29) serovar Typhimurium isolates derived from gastroenteritis patients did not possess the Salmonella virulence plasmid, in contrast to all animal and human bacteremia isolates tested. Lastly, among serovar Typhimurium isolates that harbored the Salmonella virulence plasmid, 6 of 31 derived from human salmonellosis patients were avirulent in mice, which is in contrast to the virulent phenotype exhibited by all the animal isolates examined. These studies suggest that Salmonella isolates derived from human salmonellosis patients are distinct from those of animal origin. The characterization of these bacterial strain variants may provide insight into their relative pathogenicities as well as into the development of treatment and prophylactic strategies for salmonellosis.

References

  1. Am J Vet Res. 2001 Dec;62(12):1897-902 - PubMed
  2. Infect Immun. 2001 Dec;69(12):7950-4 - PubMed
  3. J Clin Microbiol. 2003 Aug;41(8):3823-8 - PubMed
  4. Infect Immun. 1999 Nov;67(11):5651-7 - PubMed
  5. Mol Microbiol. 2000 Jun;36(5):1006-14 - PubMed
  6. Vaccine. 2003 Jul 4;21(23):3249-58 - PubMed
  7. J Am Vet Med Assoc. 2002 Mar 1;220(5):645-9 - PubMed
  8. Infect Immun. 1994 Oct;62(10):4602-10 - PubMed
  9. MMWR Morb Mortal Wkly Rep. 1995 Oct 27;44(42):785-8 - PubMed
  10. Epidemiol Infect. 1996 Aug;117(1):17-28 - PubMed
  11. J Exp Med. 1960 Jul 1;112:143-66 - PubMed
  12. Epidemiol Infect. 1990 Apr;104(2):243-51 - PubMed
  13. Appl Environ Microbiol. 2005 Mar;71(3):1616-25 - PubMed
  14. Mol Microbiol. 2001 Feb;39(3):606-19 - PubMed
  15. Genetics. 1998 Jul;149(3):1183-90 - PubMed
  16. Prev Vet Med. 2005 Jan;67(1):39-53 - PubMed
  17. Infect Immun. 1996 Jan;64(1):61-8 - PubMed
  18. Prev Vet Med. 2005 Sep 12;70(3-4):257-77 - PubMed
  19. Science. 1999 May 7;284(5416):967-70 - PubMed
  20. J Pathol Bacteriol. 1967 Jan;93(1):141-56 - PubMed
  21. Antonie Van Leeuwenhoek. 1971;37(4):473-6 - PubMed
  22. Infect Immun. 2004 Jul;72(7):4297-301 - PubMed
  23. J Bacteriol. 2001 Aug;183(15):4652-8 - PubMed
  24. Mol Microbiol. 1989 Jun;3(6):733-43 - PubMed
  25. Vet Microbiol. 2000 Apr 4;73(1):61-73 - PubMed
  26. Nat Rev Microbiol. 2004 May;2(5):414-24 - PubMed
  27. Vet Microbiol. 1995 Nov;47(1-2):43-59 - PubMed
  28. Microb Pathog. 1990 Jan;8(1):3-11 - PubMed
  29. Dtsch Tierarztl Wochenschr. 1979 Aug 5;89(8):306-7 - PubMed
  30. Infect Immun. 1994 Dec;62(12):5519-27 - PubMed
  31. Infect Immun. 1995 Jul;63(7):2755-61 - PubMed
  32. Epidemiol Infect. 2000 Oct;125(2):229-55 - PubMed
  33. Epidemiol Infect. 1996 Aug;117(1):29-34 - PubMed
  34. Microb Pathog. 1991 Jul;11(1):11-8 - PubMed
  35. Mol Microbiol. 1993 May;8(3):543-58 - PubMed
  36. J Infect Dis. 1994 Mar;169(3):547-52 - PubMed
  37. Vet Rec. 1980 Aug 30;107(9):191-3 - PubMed
  38. Mol Microbiol. 2005 Apr;56(1):1-7 - PubMed
  39. Infect Immun. 1990 May;58(5):1180-5 - PubMed
  40. Ann Inst Pasteur Microbiol. 1988 May-Jun;139(3):331-5 - PubMed
  41. J Am Vet Med Assoc. 2001 Aug 1;219(3):310-22 - PubMed
  42. Epidemiol Infect. 1987 Oct;99(2):291-4 - PubMed
  43. Infect Immun. 1996 Mar;64(3):938-44 - PubMed
  44. J Bacteriol. 1981 Mar;145(3):1365-73 - PubMed
  45. J Bacteriol. 2003 Jan;185(2):553-63 - PubMed
  46. Mol Microbiol. 1992 May;6(10):1395-411 - PubMed
  47. Proc Natl Acad Sci U S A. 1998 Apr 14;95(8):4641-5 - PubMed
  48. Berl Munch Tierarztl Wochenschr. 1971 Aug 1;84(15):290-2 - PubMed
  49. Clin Infect Dis. 2004 Apr 15;38 Suppl 3:S142-8 - PubMed
  50. Prev Vet Med. 2005 Sep 12;70(3-4):279-91 - PubMed
  51. J Dairy Res. 1977 Jun;44(2):383-425 - PubMed
  52. Mol Microbiol. 2001 Mar;39(6):1464-70 - PubMed
  53. Infect Immun. 1982 Nov;38(2):476-86 - PubMed
  54. Adv Exp Med Biol. 1999;473:261-74 - PubMed
  55. Epidemiol Infect. 1993 Jun;110(3):553-65 - PubMed
  56. Br Vet J. 1995 Jul-Aug;151(4):351-77 - PubMed
  57. Ann Rech Vet. 1988;19(4):221-35 - PubMed
  58. Microbes Infect. 2001 Mar;3(3):237-47 - PubMed
  59. Clin Infect Dis. 1997 Mar;24(3):506-10 - PubMed
  60. J Bacteriol. 2007 Mar;189(5):1556-64 - PubMed
  61. Vet Rec. 1994 May 28;134(22):577 - PubMed
  62. Zentralbl Bakteriol. 1999 Oct;289(4):399-414 - PubMed
  63. Proc Natl Acad Sci U S A. 2002 Jun 25;99(13):8956-61 - PubMed
  64. J Microbiol Methods. 2000 Mar;40(1):33-8 - PubMed
  65. Infect Immun. 2002 May;70(5):2249-55 - PubMed
  66. Epidemiol Infect. 1993 Feb;110(1):17-22 - PubMed
  67. J Hyg (Lond). 1984 Dec;93(3):439-44 - PubMed
  68. Gene. 1997 Mar 25;188(1):53-61 - PubMed
  69. Infect Immun. 2002 Jun;70(6):3290-4 - PubMed
  70. Infect Immun. 1998 Oct;66(10):4579-87 - PubMed
  71. Infect Immun. 2001 Nov;69(11):6725-30 - PubMed
  72. J Clin Microbiol. 1983 Mar;17(3):552-4 - PubMed
  73. Vet Microbiol. 2006 Jan 10;112(1):1-10 - PubMed

Substances

MeSH terms

Publication Types

Grant support