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Ledermann B, Bürki K. Establishment of a germ-line competent C57BL/6 embryonic stem cell line. Exp Cell Res. 1991;197(2):254-8doi: 10.1016/0014-4827(91)90430-3.
Ledermann, B., & Bürki, K. (1991). Establishment of a germ-line competent C57BL/6 embryonic stem cell line. Experimental cell research, 197(2), 254-8. https://doi.org/10.1016/0014-4827(91)90430-3
Ledermann, B, and Bürki, K. "Establishment of a germ-line competent C57BL/6 embryonic stem cell line." Experimental cell research vol. 197,2 (1991): 254-8. doi: https://doi.org/10.1016/0014-4827(91)90430-3
Ledermann B, Bürki K. Establishment of a germ-line competent C57BL/6 embryonic stem cell line. Exp Cell Res. 1991 Dec;197(2):254-8. doi: 10.1016/0014-4827(91)90430-3. PMID: 1959560.
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Exp Cell Res. 1991 Dec;197(2):254-8. doi: 10.1016/0014-4827(91)90430-3.

Establishment of a germ-line competent C57BL/6 embryonic stem cell line.

Experimental cell research

B Ledermann, K Bürki

Affiliations

  1. Preclinical Research, Sandoz Pharma Ltd., Basel, Switzerland.

PMID: 1959560 DOI: 10.1016/0014-4827(91)90430-3

Abstract

Embryonic stem (ES) cell lines have been derived from blastocysts of the inbred mouse strain C57BL/6. The highest frequencies of ES cell colonies were observed when blastocysts were explanted directly onto growth-arrested feeder layers of 5637 human bladder carcinoma cells in the presence of conditioned medium. One of the male ES cell lines tested (BL/6-III) was shown to be karyotypically stable and germ-line competent when introduced into BALB/c host blastocysts. These results demonstrate that ES cell lines from inbred mouse strains other than 129/Sv may be used as vectors to introduce selected mutations into the germ-line of mice.

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