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Liu Y, Shi H, Sun Z, et al. Enantiomer separation of the four diastereomers of guaiacyl glycerol from Hydnocarpus annamensis by capillary electrophoresis with HP-beta-CD as a chiral selector. J Chromatogr Sci. 2007;45(9):605-9doi: 10.1093/chromsci/45.9.605.
Liu, Y., Shi, H., Sun, Z., Ling, X., & Tu, P. (2007). Enantiomer separation of the four diastereomers of guaiacyl glycerol from Hydnocarpus annamensis by capillary electrophoresis with HP-beta-CD as a chiral selector. Journal of chromatographic science, 45(9), 605-9. https://doi.org/10.1093/chromsci/45.9.605
Liu, Yi, et al. "Enantiomer separation of the four diastereomers of guaiacyl glycerol from Hydnocarpus annamensis by capillary electrophoresis with HP-beta-CD as a chiral selector." Journal of chromatographic science vol. 45,9 (2007): 605-9. doi: https://doi.org/10.1093/chromsci/45.9.605
Liu Y, Shi H, Sun Z, Ling X, Tu P. Enantiomer separation of the four diastereomers of guaiacyl glycerol from Hydnocarpus annamensis by capillary electrophoresis with HP-beta-CD as a chiral selector. J Chromatogr Sci. 2007 Oct;45(9):605-9. doi: 10.1093/chromsci/45.9.605. PMID: 17988449.
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J Chromatogr Sci. 2007 Oct;45(9):605-9. doi: 10.1093/chromsci/45.9.605.

Enantiomer separation of the four diastereomers of guaiacyl glycerol from Hydnocarpus annamensis by capillary electrophoresis with HP-beta-CD as a chiral selector.

Journal of chromatographic science

Yi Liu, Haiming Shi, Zhe Sun, Xiaomei Ling, Pengfei Tu

Affiliations

  1. Department of Pharmaceutical Analysis, School of Pharmaceutical Sciences, Peking University, Beijing 100083, China.

PMID: 17988449 DOI: 10.1093/chromsci/45.9.605

Abstract

A capillary electrophoresis method with HP-beta-CD as the chiral selector is established for the enantioseparation of two pairs of phenylpropanoids, which are isolated from Hydnocarpus annamensis. The effects of buffer pH, HP-beta-CD and buffer concentration, applied voltage, and cartridge temperature on the enantioseparation are optimized. A baseline separation of the four diastereomers of guaiacyl glycerol is achieved in less than 10 min under these optimized conditions: 25 mmol/L Borax-NaOH buffer (pH 10.01) in the presence of 30 mmol/L HP-beta-CD at 15 degrees C and 30 kV. The experimental results show that the reported method by capillary electrophoresis for the separation of the four diastereomers of guaiacyl glycerol is powerful, sensitive, and fast, requires smaller amounts of reagents, and can be employed as a reliable alternative to other methods.

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