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Merritt ME, Harrison C, Storey C, et al. Inhibition of carbohydrate oxidation during the first minute of reperfusion after brief ischemia: NMR detection of hyperpolarized 13CO2 and H13CO3-. Magn Reson Med. 2008;60(5):1029-36doi: 10.1002/mrm.21760.
Merritt, M. E., Harrison, C., Storey, C., Sherry, A. D., & Malloy, C. R. (2008). Inhibition of carbohydrate oxidation during the first minute of reperfusion after brief ischemia: NMR detection of hyperpolarized 13CO2 and H13CO3-. Magnetic resonance in medicine, 60(5), 1029-36. https://doi.org/10.1002/mrm.21760
Merritt, Matthew E, et al. "Inhibition of carbohydrate oxidation during the first minute of reperfusion after brief ischemia: NMR detection of hyperpolarized 13CO2 and H13CO3-." Magnetic resonance in medicine vol. 60,5 (2008): 1029-36. doi: https://doi.org/10.1002/mrm.21760
Merritt ME, Harrison C, Storey C, Sherry AD, Malloy CR. Inhibition of carbohydrate oxidation during the first minute of reperfusion after brief ischemia: NMR detection of hyperpolarized 13CO2 and H13CO3-. Magn Reson Med. 2008 Nov;60(5):1029-36. doi: 10.1002/mrm.21760. PMID: 18956454; PMCID: PMC2696889.
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Magn Reson Med. 2008 Nov;60(5):1029-36. doi: 10.1002/mrm.21760.

Inhibition of carbohydrate oxidation during the first minute of reperfusion after brief ischemia: NMR detection of hyperpolarized 13CO2 and H13CO3-.

Magnetic resonance in medicine

Matthew E Merritt, Crystal Harrison, Charles Storey, A Dean Sherry, Craig R Malloy

Affiliations

  1. Advanced Imaging Research Center, University of Texas Southwestern Medical Center, Dallas, Texas, USA. [email protected]

PMID: 18956454 PMCID: PMC2696889 DOI: 10.1002/mrm.21760

Abstract

Isolated rat hearts were studied by (31)P NMR and (13)C NMR. Hyperpolarized [1-(13)C]pyruvate was supplied to control normoxic hearts and production of [1-(13)C]lactate, [1-(13)C]alanine, (13)CO(2) and H(13)CO(-) (3) was monitored with 1-s temporal resolution. Hearts were also subjected to 10 min of global ischemia followed by reperfusion. Developed pressure, heart rate, oxygen consumption, [ATP], [phosphocreatine], and pH recovered within 3 min after the ischemic period. During the first 90 s of reperfusion, [1-(13)C]alanine and [1-(13)C]lactate appeared rapidly, demonstrating metabolism of pyruvate through two enzymes largely confined to the cytosol, alanine aminotransferase, and lactate dehydrogenase. (13)CO(2) and H(13)CO(-) (3) were not detected. Late after ischemia and reperfusion, the products of pyruvate dehydrogenase, (13)CO(2) and H(13)CO(-) (3) were easily detected. Using this multinuclear NMR approach, we established that during the first 90 s of reperfusion PDH flux is essentially zero and recovers within 20 min in reversibly-injured myocardium.

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