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Ribeiro M, Straub VA, Schofield M, et al. Characterization of NO-sensitive guanylyl cyclase: expression in an identified interneuron involved in NO-cGMP-dependent memory formation. Eur J Neurosci. 2008;28(6):1157-65doi: 10.1111/j.1460-9568.2008.06416.x.
Ribeiro, M., Straub, V. A., Schofield, M., Picot, J., Benjamin, P. R., O'Shea, M., & Korneev, S. A. (2008). Characterization of NO-sensitive guanylyl cyclase: expression in an identified interneuron involved in NO-cGMP-dependent memory formation. The European journal of neuroscience, 28(6), 1157-65. https://doi.org/10.1111/j.1460-9568.2008.06416.x
Ribeiro, Maria, et al. "Characterization of NO-sensitive guanylyl cyclase: expression in an identified interneuron involved in NO-cGMP-dependent memory formation." The European journal of neuroscience vol. 28,6 (2008): 1157-65. doi: https://doi.org/10.1111/j.1460-9568.2008.06416.x
Ribeiro M, Straub VA, Schofield M, Picot J, Benjamin PR, O'Shea M, Korneev SA. Characterization of NO-sensitive guanylyl cyclase: expression in an identified interneuron involved in NO-cGMP-dependent memory formation. Eur J Neurosci. 2008 Sep;28(6):1157-65. doi: 10.1111/j.1460-9568.2008.06416.x. Epub 2008 Sep 09. PMID: 18783373.
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Eur J Neurosci. 2008 Sep;28(6):1157-65. doi: 10.1111/j.1460-9568.2008.06416.x. Epub 2008 Sep 09.

Characterization of NO-sensitive guanylyl cyclase: expression in an identified interneuron involved in NO-cGMP-dependent memory formation.

The European journal of neuroscience

Maria Ribeiro, Volko A Straub, Michael Schofield, Jo Picot, Paul R Benjamin, Michael O'Shea, Sergei A Korneev

Affiliations

  1. Department of Biology and Environmental Science, School of Life Sciences, Sussex Centre for Neuroscience, University of Sussex, Brighton, UK.

PMID: 18783373 DOI: 10.1111/j.1460-9568.2008.06416.x

Abstract

In a number of neuronal models of learning signalling by endogenous nitric oxide (NO), produced by the enzyme NO synthase (NOS), is essential for the formation of long-term memory (LTM). For example, in the molluscan model system Lymnaea, NO is required for LTM formation in the first few hours after one-trial reward conditioning. Furthermore, conditioning leads to transient up-regulation of the NOS gene in identified modulatory neurons, the cerebral giant cells (CGCs), which are known to be involved in LTM formation. In Lymnaea nothing is known however about the structure and localization of the major receptor for NO, the soluble guanylyl cyclase (sGC). Here we report on the cloning and characterization of both alpha and beta subunits of NO-sensitive sGC and show that they are coexpressed in the CGCs. Furthermore, our electrophysiological experiments on isolated CGCs show that these neurons respond to NO by generating a prolonged depolarization of the membrane potential. Moreover, we demonstrate that this depolarization is blocked by ODQ, supporting our hypothesis that it is mediated by sGC.

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