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Arnoldus EP, Dreef EJ, Noordermeer IA, et al. Feasibility of in situ hybridisation with chromosome specific DNA probes on paraffin wax embedded tissue. J Clin Pathol. 1991;44(11):900-4doi: 10.1136/jcp.44.11.900.
Arnoldus, E. P., Dreef, E. J., Noordermeer, I. A., Verheggen, M. M., Thierry, R. F., Peters, A. C., Cornelisse, C. J., Van der Ploeg, M., & Raap, A. K. (1991). Feasibility of in situ hybridisation with chromosome specific DNA probes on paraffin wax embedded tissue. Journal of clinical pathology, 44(11), 900-4. https://doi.org/10.1136/jcp.44.11.900
Arnoldus, E P, et al. "Feasibility of in situ hybridisation with chromosome specific DNA probes on paraffin wax embedded tissue." Journal of clinical pathology vol. 44,11 (1991): 900-4. doi: https://doi.org/10.1136/jcp.44.11.900
Arnoldus EP, Dreef EJ, Noordermeer IA, Verheggen MM, Thierry RF, Peters AC, Cornelisse CJ, Van der Ploeg M, Raap AK. Feasibility of in situ hybridisation with chromosome specific DNA probes on paraffin wax embedded tissue. J Clin Pathol. 1991 Nov;44(11):900-4. doi: 10.1136/jcp.44.11.900. PMID: 1752979; PMCID: PMC496626.
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J Clin Pathol. 1991 Nov;44(11):900-4. doi: 10.1136/jcp.44.11.900.

Feasibility of in situ hybridisation with chromosome specific DNA probes on paraffin wax embedded tissue.

Journal of clinical pathology

E P Arnoldus, E J Dreef, I A Noordermeer, M M Verheggen, R F Thierry, A C Peters, C J Cornelisse, M Van der Ploeg, A K Raap

Affiliations

  1. Department of Cytochemistry and Cytometry, Leiden University, The Netherlands.

PMID: 1752979 PMCID: PMC496626 DOI: 10.1136/jcp.44.11.900
Free PMC Article

Abstract

The feasibility was studied of in situ hybridisation using chromosome specific DNA probes on paraffin wax embedded normal and malignant tissues from different organs. Both isolated nuclei and 5 microns sections were used in in situ hybridisation experiments with biotinylated repetitive DNA probes specific for the centromeric regions of chromosomes 1 and 17. The hybridisation results were visualised with peroxidase-diaminobenzidine. The optimal pretreatments with sodium thiocyanate and pepsin were experimentally defined for the different tissues. Although interphase cytogenetics on paraffin wax embedded tissue is possible, the results indicate that it has its limitations, compared with investigations on fresh tumour tissue.

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