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Wittenkeller L, Mota de Freitas D, Ramasamy R. Ionophore-induced Cl- transport in human erythrocyte suspensions: a multinuclear magnetic resonance study. Biochem Biophys Res Commun. 1992;184(2):915-21doi: 10.1016/0006-291x(92)90678-e.
Wittenkeller, L., Mota de Freitas, D., & Ramasamy, R. (1992). Ionophore-induced Cl- transport in human erythrocyte suspensions: a multinuclear magnetic resonance study. Biochemical and biophysical research communications, 184(2), 915-21. https://doi.org/10.1016/0006-291x(92)90678-e
Wittenkeller, L, et al. "Ionophore-induced Cl- transport in human erythrocyte suspensions: a multinuclear magnetic resonance study." Biochemical and biophysical research communications vol. 184,2 (1992): 915-21. doi: https://doi.org/10.1016/0006-291x(92)90678-e
Wittenkeller L, Mota de Freitas D, Ramasamy R. Ionophore-induced Cl- transport in human erythrocyte suspensions: a multinuclear magnetic resonance study. Biochem Biophys Res Commun. 1992 Apr 30;184(2):915-21. doi: 10.1016/0006-291x(92)90678-e. PMID: 1575760.
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Biochem Biophys Res Commun. 1992 Apr 30;184(2):915-21. doi: 10.1016/0006-291x(92)90678-e.

Ionophore-induced Cl- transport in human erythrocyte suspensions: a multinuclear magnetic resonance study.

Biochemical and biophysical research communications

L Wittenkeller, D Mota de Freitas, R Ramasamy

Affiliations

  1. Department of Chemistry, Loyola University of Chicago, Illinois 60626.

PMID: 1575760 DOI: 10.1016/0006-291x(92)90678-e

Abstract

To investigate the effect of ionophores on Cl- distribution in human erythrocyte suspensions, we measured the membrane potential by using 19F and 31P NMR methods. Incubation of human erythrocytes with 0.005 mM of the neutral ionophores valinomycin and nonactin resulted in membrane potentials of -21.2 and -17.8 mV in the presence and absence of DIDS. However, 0.020 mM of the carboxylic ionophores lasalocid, monensin, and nigericin yielded membrane potentials similar to those measured in the absence of ionophore (-9.4 mV). In methanol, the 35Cl- NMR linewidth in the presence of valinomycin was twice as broad as those observed in the presence of carboxylic ionophores, suggesting that neutral ionophores induce Cl- efflux in part via ion pairing.

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