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Kwaambwa HM, Maikokera R. A fluorescence spectroscopic study of a coagulating protein extracted from Moringa oleifera seeds. Colloids Surf B Biointerfaces. 2007;60(2):213-20doi: 10.1016/j.colsurfb.2007.06.015.
Kwaambwa, H. M., & Maikokera, R. (2007). A fluorescence spectroscopic study of a coagulating protein extracted from Moringa oleifera seeds. Colloids and surfaces. B, Biointerfaces, 60(2), 213-20. https://doi.org/10.1016/j.colsurfb.2007.06.015
Kwaambwa, H M, and Maikokera, R. "A fluorescence spectroscopic study of a coagulating protein extracted from Moringa oleifera seeds." Colloids and surfaces. B, Biointerfaces vol. 60,2 (2007): 213-20. doi: https://doi.org/10.1016/j.colsurfb.2007.06.015
Kwaambwa HM, Maikokera R. A fluorescence spectroscopic study of a coagulating protein extracted from Moringa oleifera seeds. Colloids Surf B Biointerfaces. 2007 Nov 15;60(2):213-20. doi: 10.1016/j.colsurfb.2007.06.015. Epub 2007 Jun 21. PMID: 17646090.
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Colloids Surf B Biointerfaces. 2007 Nov 15;60(2):213-20. doi: 10.1016/j.colsurfb.2007.06.015. Epub 2007 Jun 21.

A fluorescence spectroscopic study of a coagulating protein extracted from Moringa oleifera seeds.

Colloids and surfaces. B, Biointerfaces

H M Kwaambwa, R Maikokera

Affiliations

  1. University of Botswana, Department of Chemistry, Private Bag UB 00704, Gaborone, Botswana. [email protected]

PMID: 17646090 DOI: 10.1016/j.colsurfb.2007.06.015

Abstract

The fluorescence studies of coagulating protein extracted from Moringa oleifera seeds have been studied using steady-state intrinsic fluorescence. The fluorescence spectra are dominated by tryptophan emission and the emission peak maximum (lambda(max)=343+ or -2nm) indicated that the tryptophan residue is not located in the hydrophobic core of the protein. Changes in solution pH affected the protein conformation as indicated by changes in the tryptophan fluorescence above pH 9 whereas the ionic strength had minimal effect. The exposure and environments of the tryptophan residue were determined using collisional quenchers.

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