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Justus CW, Müller HP, Simon MM, et al. Quantification of free alpha 2-macroglobulin and alpha 2-macroglobulin-protease complexes by a novel ELISA system based on streptococcal alpha 2-macroglobulin receptors. J Immunol Methods. 1990;126(1):103-8doi: 10.1016/0022-1759(90)90017-p.
Justus, C. W., Müller, H. P., Simon, M. M., & Kramer, M. D. (1990). Quantification of free alpha 2-macroglobulin and alpha 2-macroglobulin-protease complexes by a novel ELISA system based on streptococcal alpha 2-macroglobulin receptors. Journal of immunological methods, 126(1), 103-8. https://doi.org/10.1016/0022-1759(90)90017-p
Justus, C W, et al. "Quantification of free alpha 2-macroglobulin and alpha 2-macroglobulin-protease complexes by a novel ELISA system based on streptococcal alpha 2-macroglobulin receptors." Journal of immunological methods vol. 126,1 (1990): 103-8. doi: https://doi.org/10.1016/0022-1759(90)90017-p
Justus CW, Müller HP, Simon MM, Kramer MD. Quantification of free alpha 2-macroglobulin and alpha 2-macroglobulin-protease complexes by a novel ELISA system based on streptococcal alpha 2-macroglobulin receptors. J Immunol Methods. 1990 Jan 24;126(1):103-8. doi: 10.1016/0022-1759(90)90017-p. PMID: 1689357.
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J Immunol Methods. 1990 Jan 24;126(1):103-8. doi: 10.1016/0022-1759(90)90017-p.

Quantification of free alpha 2-macroglobulin and alpha 2-macroglobulin-protease complexes by a novel ELISA system based on streptococcal alpha 2-macroglobulin receptors.

Journal of immunological methods

C W Justus, H P Müller, M M Simon, M D Kramer

Affiliations

  1. MPG Klinische Forschungsgruppe für Blutgerinnung und Thrombose, Giessen, F.R.G.

PMID: 1689357 DOI: 10.1016/0022-1759(90)90017-p

Abstract

An ELISA test system has been developed for the quantification of the two distinct forms of the proteinase inhibitor alpha 2-macroglobulin (alpha 2M): (i) free alpha 2M (functionally active), which is the electrophoretically slow form (alpha 2 MS), and (ii) the alpha 2 M-proteinase complex (functionally inactive), which is the electrophoretically fast form of alpha 2 M (alpha 2 MF). Discrimination between the two types of alpha 2 M was achieved using extracts of the two independent streptococcal strains, M1 and Sc1, which express receptors for alpha 2 MS and alpha 2 MF, respectively, in combination with a monoclonal antibody specific for alpha 2 M. The assay system described is easy and reliable and permits quantitation of alpha 2 MS and alpha 2 MF in complex biological samples such as plasma and cutaneous suction blister fluid.

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