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Ishikawa TO, Herschman HR. Two inducible, functional cyclooxygenase-2 genes are present in the rainbow trout genome. J Cell Biochem. 2007;102(6):1486-92doi: 10.1002/jcb.21368.
Ishikawa, T. O., & Herschman, H. R. (2007). Two inducible, functional cyclooxygenase-2 genes are present in the rainbow trout genome. Journal of cellular biochemistry, 102(6), 1486-92. https://doi.org/10.1002/jcb.21368
Ishikawa, Tomo-o, and Herschman, Harvey R. "Two inducible, functional cyclooxygenase-2 genes are present in the rainbow trout genome." Journal of cellular biochemistry vol. 102,6 (2007): 1486-92. doi: https://doi.org/10.1002/jcb.21368
Ishikawa TO, Herschman HR. Two inducible, functional cyclooxygenase-2 genes are present in the rainbow trout genome. J Cell Biochem. 2007 Dec 15;102(6):1486-92. doi: 10.1002/jcb.21368. PMID: 17471498.
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J Cell Biochem. 2007 Dec 15;102(6):1486-92. doi: 10.1002/jcb.21368.

Two inducible, functional cyclooxygenase-2 genes are present in the rainbow trout genome.

Journal of cellular biochemistry

Tomo-o Ishikawa, Harvey R Herschman

Affiliations

  1. Department of Molecular and Medical Pharmacology, David Geffen School of Medicine, UCLA, Los Angeles, California 90095, USA.

PMID: 17471498 DOI: 10.1002/jcb.21368

Abstract

The cyclooxygenases (Cox) catalyze the initial reactions in prostanoid biosynthesis, and produce the common prostanoids precursor, PGH(2). Mammalian species have two Cox isoforms; constitutively expressed cyclooxygenase-1 (Cox-1) and inducible cyclooxygenase-2 (Cox-2). Database searches suggest three Cox genes are present in many fish species. In this study, we cloned and characterized a second Cox-2 cDNA, Cox-2b, from the rainbow trout. Rainbow trout Cox-2b protein contains all the functionally important conserved amino acids for Cox enzyme activity. Moreover, the Cox-2b message contains AU-rich elements (AREs) in the 3' untranslated region (3'UTR) characteristic of inducible Cox-2 mRNAs. We took advantage of the existence of a rainbow trout cell line to demonstrate that expression from both the originally reported Cox-2 (Cox-2a) and Cox-2b genes is inducible. However, differential induction responses to alternative inducers are observed for rainbow trout Cox-2a and Cox-2b. Both Cox-2a and Cox-2b proteins expressed in COS cells are enzymatically active. Thus the rainbow trout has two functional, inducible Cox-2 genes. The zebrafish also contains two Cox-2 genes. However, genome structure analysis suggests diversion of the Cox-2a gene between zebrafish and rainbow trout.

Copyright (c) 2007 Wiley-Liss, Inc.

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