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Guisasola MC, Desco Mdel M, Gonzalez FS, et al. Heat shock proteins, end effectors of myocardium ischemic preconditioning?. Cell Stress Chaperones. 2006;11(3):250-8doi: 10.1379/csc-181r1.1.
Guisasola, M. C., Desco, M. d. e. l. . M., Gonzalez, F. S., Asensio, F., Dulin, E., Suarez, A., & Garcia Barreno, P. (2006). Heat shock proteins, end effectors of myocardium ischemic preconditioning?. Cell stress & chaperones, 11(3), 250-8. https://doi.org/10.1379/csc-181r1.1
Guisasola, María Concepcion, et al. "Heat shock proteins, end effectors of myocardium ischemic preconditioning?." Cell stress & chaperones vol. 11,3 (2006): 250-8. doi: https://doi.org/10.1379/csc-181r1.1
Guisasola MC, Desco Mdel M, Gonzalez FS, Asensio F, Dulin E, Suarez A, Garcia Barreno P. Heat shock proteins, end effectors of myocardium ischemic preconditioning?. Cell Stress Chaperones. 2006;11(3):250-8. doi: 10.1379/csc-181r1.1. PMID: 17009598; PMCID: PMC1576469.
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Cell Stress Chaperones. 2006;11(3):250-8. doi: 10.1379/csc-181r1.1.

Heat shock proteins, end effectors of myocardium ischemic preconditioning?.

Cell stress & chaperones

María Concepcion Guisasola, Maria del Mar Desco, Fernanda Silvana Gonzalez, Fernando Asensio, Elena Dulin, Antonio Suarez, Pedro Garcia Barreno

Affiliations

  1. Unit of Experimental Medicine and Surgery, Hospital General Universitario Gregorio Marañon, 28007 Madrid, Spain. [email protected]

PMID: 17009598 PMCID: PMC1576469 DOI: 10.1379/csc-181r1.1

Abstract

The purpose of this study was to investigate (1) whether ischemia-reperfusion increased the content of heat shock protein 72 (Hsp72) transcripts and (2) whether myocardial content of Hsp72 is increased by ischemic preconditioning so that they can be considered as end effectors of preconditioning. Twelve male minipigs (8 protocol, 4 sham) were used, with the following ischemic preconditioning protocol: 3 ischemia and reperfusion 5-minute alternative cycles and last reperfusion cycle of 3 hours. Initial and final transmural biopsies (both in healthy and ischemic areas) were taken in all animals. Heat shock protein 72 messenger ribonucleic acid (mRNA) expression was measured by a semiquantitative reverse transcriptase-polymerase chain reaction (RT-PCR) method using complementary DNA normalized against the housekeeping gene cyclophilin. The identification of heat shock protein 72 was performed by immunoblot. In our "classic" preconditioning model, we found no changes in mRNA hsp72 levels or heat shock protein 72 content in the myocardium after 3 hours of reperfusion. Our experimental model is valid and the experimental techniques are appropriate, but the induction of heat shock proteins 72 as end effectors of cardioprotection in ischemic preconditioning does not occur in the first hours after ischemia, but probably at least 24 hours after it, in the so-called "second protection window."

References

  1. Proc Natl Acad Sci U S A. 1996 Mar 19;93(6):2339-42 - PubMed
  2. J Clin Invest. 1995 Apr;95(4):1854-60 - PubMed
  3. Cardiovasc Res. 2001 Sep;51(4):637-46 - PubMed
  4. Cardiovasc Res. 2004 Feb 15;61(3):414-26 - PubMed
  5. Nature. 1970 Aug 15;227(5259):680-5 - PubMed
  6. Jpn Circ J. 1998 Jul;62(7):512-6 - PubMed
  7. Cardiovasc Res. 2004 Feb 15;61(3):402-13 - PubMed
  8. Circulation. 2001 Nov 27;104(22):E123 - PubMed
  9. J Appl Physiol (1985). 2003 May;94(5):1689-701 - PubMed
  10. EMBO J. 2003 Aug 1;22(15):3783-91 - PubMed
  11. Cardiovasc Res. 2004 Feb 15;61(3):437-47 - PubMed
  12. Cell Stress Chaperones. 2005 Spring;10(1):17-23 - PubMed
  13. Cardiovasc Res. 2004 Feb 15;61(3):461-70 - PubMed
  14. Physiol Rev. 2003 Oct;83(4):1113-51 - PubMed
  15. Biol Proced Online. 2003;5:20-28 - PubMed
  16. J Mol Cell Cardiol. 1995 Oct;27(10):2133-48 - PubMed
  17. Circ Res. 1988 Sep;63(3):512-7 - PubMed
  18. Transplantation. 1976 Dec;22(6):559-67 - PubMed
  19. Circulation. 1983 Aug;68(2 Pt 2):I25-36 - PubMed
  20. Am J Physiol. 1991 Mar;260(3 Pt 2):H796-804 - PubMed
  21. Cardiovasc Res. 2003 Dec 1;60(3):469-77 - PubMed
  22. Circulation. 1993 Sep;88(3):1264-72 - PubMed
  23. J Surg Res. 2003 Jan;109(1):24-30 - PubMed
  24. Cell Stress Chaperones. 1997 Mar;2(1):1-11 - PubMed
  25. EXS. 1996;77:121-37 - PubMed
  26. Cell Mol Biol (Noisy-le-grand). 1992 Nov;38(7):739-49 - PubMed
  27. Eur J Cancer. 1994;30A(12):1884-91 - PubMed
  28. Cardiovasc Res. 2004 Feb 15;61(3):427-36 - PubMed
  29. Basic Res Cardiol. 2004 Nov;99(6):392-4 - PubMed
  30. Adv Exp Med Biol. 2003;543:277-91 - PubMed
  31. J Surg Res. 2002 Jun 15;105(2):248-58 - PubMed
  32. Basic Res Cardiol. 1997 Jun;92(3):159-67 - PubMed
  33. Pharmacol Ther. 1999 Jul;83(1):21-37 - PubMed
  34. Circulation. 2002 Feb 26;105(8):1012-7 - PubMed
  35. Circ Res. 2000 May 12;86(9):989-97 - PubMed
  36. Circulation. 1996 Sep 15;94(6):1408-11 - PubMed
  37. Experientia. 1992 Jul 15;48(7):629-34 - PubMed
  38. Science. 2002 Mar 8;295(5561):1852-8 - PubMed
  39. Pharmacol Ther. 2003 Jan;97(1):35-53 - PubMed
  40. Am J Physiol Heart Circ Physiol. 2002 Mar;282(3):H935-41 - PubMed
  41. Cardiovasc Res. 1993 Apr;27(4):551-5 - PubMed
  42. Am J Physiol. 1995 Jan;268(1 Pt 2):H233-41 - PubMed
  43. J Clin Invest. 1995 Jan;95(1):388-403 - PubMed
  44. Immunol Today. 1988 May;9(5):134-7 - PubMed
  45. J Biol Chem. 1951 Nov;193(1):265-75 - PubMed
  46. J Thorac Cardiovasc Surg. 2003 Mar;125(3):678-85 - PubMed
  47. Arterioscler Thromb Vasc Biol. 2002 Oct 1;22(10):1547-59 - PubMed
  48. J Clin Invest. 1991 Jan;87(1):139-47 - PubMed
  49. Circulation. 1986 Nov;74(5):1124-36 - PubMed
  50. Trends Immunol. 2002 Mar;23(3):130-5 - PubMed
  51. J Mol Biol. 1974 Apr 15;84(3):389-98 - PubMed
  52. J Clin Invest. 1995 Apr;95(4):1446-56 - PubMed
  53. Cardiovasc Res. 2004 Feb 15;61(3):363-4 - PubMed
  54. Arterioscler Thromb Vasc Biol. 2001 Mar;21(3):321-6 - PubMed
  55. Essays Biochem. 1997;32:17-29 - PubMed
  56. J Mol Cell Cardiol. 1994 Jun;26(6):695-9 - PubMed
  57. Biotechniques. 1993 Sep;15(3):532-4, 536-7 - PubMed

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