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Appl Environ Microbiol. 2006 Sep;72(9):6111-6. doi: 10.1128/AEM.02580-05.

Visible-light-induced bactericidal activity of a nitrogen-doped titanium photocatalyst against human pathogens.

Applied and environmental microbiology

Ming-Show Wong, Wen-Chen Chu, Der-Shan Sun, Hsuan-Shun Huang, Jiann-Hwa Chen, Pei-Jane Tsai, Nien-Tsung Lin, Mei-Shiuan Yu, Shang-Feng Hsu, Shih-Lien Wang, Hsin-Hou Chang

Affiliations

  1. Department of Materials Science and Engineering, National Dong-Hwa University, Hualien, Taiwan.

PMID: 16957236 PMCID: PMC1563686 DOI: 10.1128/AEM.02580-05

Abstract

The antibacterial activity of photocatalytic titanium dioxide (TiO(2)) substrates is induced primarily by UV light irradiation. Recently, nitrogen- and carbon-doped TiO(2) substrates were shown to exhibit photocatalytic activities under visible-light illumination. Their antibacterial activity, however, remains to be quantified. In this study, we demonstrated that nitrogen-doped TiO(2) substrates have superior visible-light-induced bactericidal activity against Escherichia coli compared to pure TiO(2) and carbon-doped TiO(2) substrates. We also found that protein- and light-absorbing contaminants partially reduce the bactericidal activity of nitrogen-doped TiO(2) substrates due to their light-shielding effects. In the pathogen-killing experiment, a significantly higher proportion of all tested pathogens, including Shigella flexneri, Listeria monocytogenes, Vibrio parahaemolyticus, Staphylococcus aureus, Streptococcus pyogenes, and Acinetobacter baumannii, were killed by visible-light-illuminated nitrogen-doped TiO(2) substrates than by pure TiO(2) substrates. These findings suggest that nitrogen-doped TiO(2) has potential application in the development of alternative disinfectants for environmental and medical usages.

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