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Voha C, Docquier JD, Rossolini GM, et al. Genetic and biochemical characterization of FUS-1 (OXA-85), a narrow-spectrum class D beta-lactamase from Fusobacterium nucleatum subsp. polymorphum. Antimicrob Agents Chemother. 2006;50(8):2673-9doi: 10.1128/AAC.00058-06.
Voha, C., Docquier, J. D., Rossolini, G. M., & Fosse, T. (2006). Genetic and biochemical characterization of FUS-1 (OXA-85), a narrow-spectrum class D beta-lactamase from Fusobacterium nucleatum subsp. polymorphum. Antimicrobial agents and chemotherapy, 50(8), 2673-9. https://doi.org/10.1128/AAC.00058-06
Voha, Christine, et al. "Genetic and biochemical characterization of FUS-1 (OXA-85), a narrow-spectrum class D beta-lactamase from Fusobacterium nucleatum subsp. polymorphum." Antimicrobial agents and chemotherapy vol. 50,8 (2006): 2673-9. doi: https://doi.org/10.1128/AAC.00058-06
Voha C, Docquier JD, Rossolini GM, Fosse T. Genetic and biochemical characterization of FUS-1 (OXA-85), a narrow-spectrum class D beta-lactamase from Fusobacterium nucleatum subsp. polymorphum. Antimicrob Agents Chemother. 2006 Aug;50(8):2673-9. doi: 10.1128/AAC.00058-06. PMID: 16870757; PMCID: PMC1538689.
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Antimicrob Agents Chemother. 2006 Aug;50(8):2673-9. doi: 10.1128/AAC.00058-06.

Genetic and biochemical characterization of FUS-1 (OXA-85), a narrow-spectrum class D beta-lactamase from Fusobacterium nucleatum subsp. polymorphum.

Antimicrobial agents and chemotherapy

Christine Voha, Jean-Denis Docquier, Gian Maria Rossolini, Thierry Fosse

Affiliations

  1. Laboratoire de Bactériologie et Epidémiologie Moléculaire, Université de Nice, Centre Hospitalier Universitaire de Nice, France.

PMID: 16870757 PMCID: PMC1538689 DOI: 10.1128/AAC.00058-06

Abstract

Previous studies have reported beta-lactamase-mediated penicillin resistance in Fusobacterium nucleatum, but no beta-lactamase gene has yet been identified in this species. An F. nucleatum subsp. polymorphum strain resistant to penicillin and amoxicillin was isolated from a human periodontitis sample. DNA cloning and sequencing revealed a 765-bp open reading frame encoding a new class D beta-lactamase named FUS-1 (OXA-85). A recombinant Escherichia coli strain carrying the bla(FUS-1) gene exhibited resistance to amoxicillin with a moderate decrease in the MICs with clavulanic acid. The bla(FUS-1) gene was found in two additional clonally unrelated F. nucleatum subsp. polymorphum isolates. It was located on the chromosome in a peculiar genetic environment where a gene encoding a putative transposase-like protein is found, suggesting a possible acquisition of this class D beta-lactamase gene. The FUS-1 enzyme showed the closest ancestral relationship with OXA-63 from Brachyspira pilosicoli (53% identity) and with putative chromosomal beta-lactamases of Campylobacter spp. (40 to 42% identity). FUS-1 presents all of the conserved structural motifs of class D beta-lactamases. Kinetic analysis revealed that FUS-1 exhibits a narrow substrate profile, efficiently hydrolyzing benzylpenicillin and oxacillin. FUS-1 was poorly inactivated by clavulanate and NaCl. FUS-1 is the first example of a class D beta-lactamase produced by a gram-negative, anaerobic, rod-shaped bacterium to be characterized.

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