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Ji XM, Xie CH, Fang MH, et al. Efficient inhibition of human telomerase activity by antisense oligonucleotides sensitizes cancer cells to radiotherapy. Acta Pharmacol Sin. 2006;27(9):1185-91doi: 10.1111/j.1745-7254.2006.00417.x.
Ji, X. M., Xie, C. H., Fang, M. H., Zhou, F. X., Zhang, W. J., Zhang, M. S., & Zhou, Y. F. (2006). Efficient inhibition of human telomerase activity by antisense oligonucleotides sensitizes cancer cells to radiotherapy. Acta pharmacologica Sinica, 27(9), 1185-91. https://doi.org/10.1111/j.1745-7254.2006.00417.x
Ji, Xue-mei, et al. "Efficient inhibition of human telomerase activity by antisense oligonucleotides sensitizes cancer cells to radiotherapy." Acta pharmacologica Sinica vol. 27,9 (2006): 1185-91. doi: https://doi.org/10.1111/j.1745-7254.2006.00417.x
Ji XM, Xie CH, Fang MH, Zhou FX, Zhang WJ, Zhang MS, Zhou YF. Efficient inhibition of human telomerase activity by antisense oligonucleotides sensitizes cancer cells to radiotherapy. Acta Pharmacol Sin. 2006 Sep;27(9):1185-91. doi: 10.1111/j.1745-7254.2006.00417.x. PMID: 16923339.
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Acta Pharmacol Sin. 2006 Sep;27(9):1185-91. doi: 10.1111/j.1745-7254.2006.00417.x.

Efficient inhibition of human telomerase activity by antisense oligonucleotides sensitizes cancer cells to radiotherapy.

Acta pharmacologica Sinica

Xue-mei Ji, Cong-hua Xie, Ming-hao Fang, Fu-xiang Zhou, Wen-jie Zhang, Ming-sheng Zhang, Yun-feng Zhou

Affiliations

  1. Department of Chemotherapy and Radiation Oncology, Zhongnan Hospital, Wuhan University, Wuhan 430071, China.

PMID: 16923339 DOI: 10.1111/j.1745-7254.2006.00417.x

Abstract

AIM: To investigate the effect of the antisense oligonucleotides (ASODN) specific for human telomerase RNA (hTR) on radio sensitization and proliferation inhibition in human neurogliocytoma cells (U251).

METHODS: U251 cells were transfected with hTR ASODN or nonspecific oligonucleotides (NSODN). Before and after irradiation of (60)Co- gamma ray, telomerase activity was assayed by telomeric repeat amplification protocol ( TRAP-PCR-ELISA), and DNA damage and repair were examined by the comet assay. The classical colony assay was used to plot the cell-survival curve, to detect the D(0 )value.

RESULTS: hTR antisense oligonucleotides could downregulate the telomerase activity, increase radiation induced DNA damage and reduce the subsequent repair. Furthermore, it could inhibit the proliferation and decrease the D(0 ) value which demonstrates rising radiosensitivity. However, telomere length was unchanged over a short period of time.

CONCLUSION: These findings suggest that an ASODN-based strategy may be used to develop telomerase inhibitors, which can efficiently sensitize radiotherapy.

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