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Oldham CD, Li C, Girard PR, et al. Peptide amidating enzymes are present in cultured endothelial cells. Biochem Biophys Res Commun. 1992;184(1):323-9doi: 10.1016/0006-291x(92)91196-w.
Oldham, C. D., Li, C., Girard, P. R., Nerem, R. M., & May, S. W. (1992). Peptide amidating enzymes are present in cultured endothelial cells. Biochemical and biophysical research communications, 184(1), 323-9. https://doi.org/10.1016/0006-291x(92)91196-w
Oldham, C D, et al. "Peptide amidating enzymes are present in cultured endothelial cells." Biochemical and biophysical research communications vol. 184,1 (1992): 323-9. doi: https://doi.org/10.1016/0006-291x(92)91196-w
Oldham CD, Li C, Girard PR, Nerem RM, May SW. Peptide amidating enzymes are present in cultured endothelial cells. Biochem Biophys Res Commun. 1992 Apr 15;184(1):323-9. doi: 10.1016/0006-291x(92)91196-w. PMID: 1567439.
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Elsevier Science

Biochem Biophys Res Commun. 1992 Apr 15;184(1):323-9. doi: 10.1016/0006-291x(92)91196-w.

Peptide amidating enzymes are present in cultured endothelial cells.

Biochemical and biophysical research communications

C D Oldham, C Li, P R Girard, R M Nerem, S W May

Affiliations

  1. School of Chemistry & Biochemistry, Georgia Institute of Technology, Atlanta 30332.

PMID: 1567439 DOI: 10.1016/0006-291x(92)91196-w

Abstract

Carboxy-terminal amidation is a prevalent post-translational modification necessary for the bioactivity of many peptides. We now report that the two enzymes essential for amidation, peptidylglycine alpha-monooxygenase (PAM) and peptidylamidoglycolate lyase (PGL), are present in both the cytosol and membrane fractions of cultured bovine aortic endothelial cells. Endothelial PAM exhibits ascorbate-dependent turnover and is inactivated by the mechanism-based inactivator, 4-phenyl-3-butenoic acid (PBA), whereas PGL activity is independent of ascorbate and is not affected by PBA. These enzymological characteristics correspond to those of amidating enzymes from other tissues. These results suggest a heretofore unrecognized role for alpha-amidated peptides in cardiovascular function.

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