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Braulke T, Bresciani R, Buergisser DM, et al. Insulin-like growth factor II overexpression does not affect sorting of lysosomal enzymes in NIH-3T3 cells. Biochem Biophys Res Commun. 1991;179(1):108-15doi: 10.1016/0006-291x(91)91341-9.
Braulke, T., Bresciani, R., Buergisser, D. M., & von Figura, K. (1991). Insulin-like growth factor II overexpression does not affect sorting of lysosomal enzymes in NIH-3T3 cells. Biochemical and biophysical research communications, 179(1), 108-15. https://doi.org/10.1016/0006-291x(91)91341-9
Braulke, T, et al. "Insulin-like growth factor II overexpression does not affect sorting of lysosomal enzymes in NIH-3T3 cells." Biochemical and biophysical research communications vol. 179,1 (1991): 108-15. doi: https://doi.org/10.1016/0006-291x(91)91341-9
Braulke T, Bresciani R, Buergisser DM, von Figura K. Insulin-like growth factor II overexpression does not affect sorting of lysosomal enzymes in NIH-3T3 cells. Biochem Biophys Res Commun. 1991 Aug 30;179(1):108-15. doi: 10.1016/0006-291x(91)91341-9. PMID: 1679327.
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Elsevier Science

Biochem Biophys Res Commun. 1991 Aug 30;179(1):108-15. doi: 10.1016/0006-291x(91)91341-9.

Insulin-like growth factor II overexpression does not affect sorting of lysosomal enzymes in NIH-3T3 cells.

Biochemical and biophysical research communications

T Braulke, R Bresciani, D M Buergisser, K von Figura

Affiliations

  1. Georg-August-Universität, Biochemie II, Göttingen, Germany.

PMID: 1679327 DOI: 10.1016/0006-291x(91)91341-9

Abstract

The sorting of newly synthesized mannose 6-phosphate (M6P)-containing proteins and of the major excreted protein (MEP), a lysosomal thiol proteinase, was studied in NIH-3T3 cells transfected with the cDNA of human insulin-like growth factor II (IGF II) or with the vector alone. Extracts from media and cells labelled with [35S] methionine were used for chromatography on a M6P/IGF II receptor affinity matrix or for immunoprecipitation to assess the distribution of newly synthesized M6P-containing proteins and MEP, respectively. The results indicate that the overexpression of IGF II did not affect the synthesis and the sorting of M6P-containing proteins and of MEP. The binding and uptake of the lysosomal enzyme arylsulfatase A were not affected in IGF II overexpressing cells.

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